主页 > 医学讨论 >
【medical-news】艾滋病的基因疗法
Main Category: HIV / AIDS News
Article Date: 29 Jan 2007 - 0:00 PST
Specialists of the V.A. Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, and the "Vector" Main Research Center of Virology and Biotechnology created and tested on the cell culture three genetic structures capable to suppress reproduction of human immunodeficiency virus (HIV-1) in human cells.
At present, the virus is fought against by chemical agents. The drugs subscribed to the patients act predominantly on key HIV-1 enzymes - reverse transcriptase, invertase and protease. There are a lot of antiviral drugs, but they are often ineffective as HIV mutates quickly and acquires drug resistance. And these drugs are, one should note, toxic and very expensive.
Meanwhile, the human organism's cells possess powerful natural mechanism which should regulate the work of genes including viral ones. It is called RNA-interference. In an extremely simplified form, RNA-interference is damage to a certain RNA sequence with participation of a different, "defending" RNA molecule. This system prevents viral infection, unless viruses had learned to cut it off in the course of evolution. Researchers from countries including Russia are developing the artificial RNA-interference system. It is non-injurious to the patient and, due to high specificity of action, does not damage its own RNA in cells infected by the virus.
To fight against HIV, Russian biologists have created three genetic structures. These structures contain short nucleotide sequences that find the most conservative molecules among all RNA molecules, that is, sequences that do not change quickly and are important to the virus. These sequences are then "damaged". The structure also includes the gene of green fluorescent protein, which allows to determine is the gene structure has entered the cell or not.
The researchers embedded the gene structures created by them into cultivated lymphoid cells. Cells which have been penetrated by the fluorescent protein begin to glow with green. Within 24 and 72 hours after introduction of genetic structures, the cells were infected by human immunodeficiency virus (GKV-4046 culture), and several days later the researchers assessed the degree of viral welfare by specific antigen accumulation. It has turned out that the genetic structures significantly suppress viral reproduction.
The extent of damage to viral RNA depends on the viral dose received by the cell itself and on the sequence of the structure per se. The sequence aimed at the reverse transcriptase area of viral genome turned out to be the most efficient, being capable of suppressing the viral production in the cells by 91 to 98 percent within three days.
In the researchers' opinion, similar genetic structures can be used in AIDS gene therapy. At present, the researchers continue the effort on creation of efficiently operating structures, including the ones that are able to overcome high virus mutation.
http://www.medicalnewstoday.com/medicalnews.php?newsid=61807 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Gene Therapy Of AIDS
艾滋病的基因疗法
Main Category: HIV / AIDS News
主要类别：艾滋病新闻
Article Date: 29 Jan 2007 - 0:00 PST
文章日期：2007年1月29号
Specialists of the V.A. Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, and the "Vector" Main Research Center of Virology and Biotechnology created and tested on the cell culture three genetic structures capable to suppress reproduction of human immunodeficiency virus (HIV-1) in human cells.
俄罗斯科学院V.A. Engelgardt分子生物学研究所及病毒生物工程学研究中心的科学家们在培养的人类细胞中设计检测出了能抑制人类免疫缺陷病毒（HIV-1）复制繁殖的三种基因片断。
At present, the virus is fought against by chemical agents. The drugs subscribed to the patients act predominantly on key HIV-1 enzymes - reverse transcriptase, invertase and protease. There are a lot of antiviral drugs, but they are often ineffective as HIV mutates quickly and acquires drug resistance. And these drugs are, one should note, toxic and very expensive.
目前，艾滋病还是依靠化学药物进行治疗。这些药物大部分作用于相关的酶类，包括逆转录酶、转化酶及蛋白酶等。虽然抗病毒药物有很多，但是当HIV-1很快发生变异或产生耐药性时，它们通常都失去了作用。而且，需要注意的是这些药物通常具有毒性且价格昂贵。
Meanwhile, the human organism's cells possess powerful natural mechanism which should regulate the work of genes including viral ones. It is called RNA-interference. In an extremely simplified form, RNA-interference is damage to a certain RNA sequence with participation of a different, "defending" RNA molecule. This system prevents viral infection, unless viruses had learned to cut it off in the course of evolution. Researchers from countries including Russia are developing the artificial RNA-interference system. It is non-injurious to the patient and, due to high specificity of action, does not damage its own RNA in cells infected by the virus.
阅读本文的人还阅读：
作者:admin@医学,生命科学 2011-08-24 05:12
医学,生命科学网