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紫杉醇分析文献翻译
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前面的摘要翻译如下：
Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana
Abstract
A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV,
1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2’-deacetoxydecinnamoyltaxinine J and 2’-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV–Vis as well as photodiode array detection.
Ó 1999 Elsevier Science B.V. All rights reserved.
Keywords: Taxus wallichiana; Plant materials; Taxol; Taxoids
1. Introduction
Taxol, a promising anti-cancer drug, is in great demand for clinical use [1,2]. This has led to the discovery of alternative sources of taxol. A number of Taxus spp. as well as their cell cultures have been subjected to intensive research in the search for this drug [2–18]. Besides, efforts are made to find alternative analogues from more common Taxus species which may have improved activity, less toxicity and better solubility in water. As part of these studies, a number of compounds were isolated and characterised from Taxus wallichiana at this institute [15–17].
A search of the literature shows that for quick screening of taxol and related taxanes in plant extracts as well as in cell cultures, reversed-phase column liquid chromatography (RPLC) has been employed using several stationary and mobile phases, detection modes, extraction procedures and techniques. A number of high-performance liquid chromatography (HPLC) analyses are reported for the analysis of plant material or cell cultures of T. brevilofolia, T. baccata, T. canadensis, T. cuspidata, T.X media, T. X media Nigra, T. X media Hicksii, T. X media Densiformis, T. cuspidata capitata, T. chinensis, T. floridana, T. yunnanensis [3,4,18–27],but none of these deal with the chemical analysis of T. wallichiana. Most of the HPLC analyses reported are related to the analysis of taxol and its precursors, cephalomannine and its precursors, 10-deacetylbac- III and baccatin III, but none of them deal with the identification of other taxoids present in the needles of these plants (Fig. 1). The possible coelution of cinnamoyl taxanes with taxol was taken into account by different workers [11,20,23,24]. In the present study, a reliable, simple and reproducible method for the extraction of taxol and other taxoids and their quantitative estimation in the needles of T. wallichiana has been developed. The analyses were performed on Symmetry C , Curosil-B and Nova-Pak Phenyl columns, using different mobile phases and gradient profiles. It was found that the best resolution was achieved on the Nova-Pak Phenyl column for these compounds. Photodiode array (PDA) detection was also used for the peak identification, peak homogeneity and peak purity of each individual compound by comparing the PDA-generated UV spectra with those of reference compounds using library matching as the retention times and spiking technique may lead to ambiguous assigncatinments.
2. Experimental
2.1. Chemicals and reagents
All the standards of taxol, 10-deacetylbaccatin III (10-DAB III), baccatin IV, 1b-hydroxybaccatin I, brevifoliol, 2-acetoxybrevifoliol, 29-deacetoxytax- 18 inine J and 29-deacetyldecinnamoyltaxinine J were isolated and characterised at CIMAP [15–17]. The purity of each compound was ascertained by HPLC–PDA. The 10-DAB III and taxol were 98% pure. Methanol and acetonitrile used were of HPLC-grade (Omnisolv, EM Science, USA). The distilled water was prepared from deionised water which was subjected to double distillation through a quartz distillation apparatus at the institute. The water was filtered through a 0.45-mm filter before use.
2.2. Equipment
HPLC analysis was done on a Waters modular system consisting of two 501 pumps, an automated gradient controller, a U6K injector, an in-line degasser, a 484 tunable absorbance detector, a 996 photodiode array detector and a Millennium 2010 chromatography manager. The injector, gradient controller and Millennium 2010 chromatography manager were integrated to give reproducible results. Nova-Pak C (4 mm, 15033.9 mm, Waters, USA), 18 Symmetry C (5 mm, 15033.9 mm,Waters), Nova- 18 Pak Phenyl (4 mm, 15033.9 mm, Waters) and Curosil-B (3 mm, 25034.6 mm, Phenomenex, USA) columns were used for the analysis and the spectral analysis was carried out at 228 nm.
摘要 ：
利用一种Nova-Pak苯基柱与二元梯度洗脱的反相液相色谱对红豆杉树叶中的紫杉醇、10-去乙酰巴卡亭III、巴卡亭IV、1-羟基巴卡亭I、2-乙酰氧短叶醇、短叶醇、2’-去乙酰苯乙烯醛基紫杉碱J（2’-去乙酰肉桂酰紫杉碱J）、2’-去乙酰氧紫杉碱J进行了分析。利用紫外-可见风光光度计对多项分析参数如提取效率、检测限、重现性及峰纯度进行了验证。
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作者:admin@医学,生命科学 2011-08-12 05:32
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