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【科普】新生物传感器可检测细菌生长及药敏性
科学家将这种装置称为“异步磁珠转动（AMBR）传感器”，它采用了一种可以在磁场中异步旋转的磁性小珠，任何附着到这种磁珠的物质都会降低其转速。在这项研究中，研究人员将杆状大肠杆菌附着在磁珠上，然后用AMBR传感器进行检测。
“当单个细菌附着上去后……将极大地阻碍磁珠，使磁珠旋转速率减慢到原来的四分之一”，领导这项研究的Raoul Kopelman教授解释，“若细菌再长大一点点，阻碍力将持续增大，转速也将随之变化，因而我们可测量出细菌的这种纳米级生长变化”。
利用同样的原理，该装置也可用于检测细菌的药敏性。当细菌受到药物影响停止持续生长，进而使得磁珠转速发生变化，于是研究人员便能在数分钟内知道药物是否对细菌产生了作用。
“采用这种方法，我们可以检测到小至80纳米程度的细菌生长变化，远比一台光学显微镜管用——显微镜的解析度也就大约250纳米”，文章第一作者Paivo Kinnunen说，“这种方法可以应用到任何微米级或纳米级的大小变化检测中”。
研究人员表示，这种新型生物传感装置或将有助于加快细菌感染治疗。（科学网 张笑/编译）
相关仪器：IX71型倒置光学显微镜 异步磁珠转动传感器
完成人：拉乌尔·科普曼课题组
实验室：美国密歇根大学化学系、生物医药工程系、化学工程系、病理学系、应用物理计划兰道实验室 密歇根大学卫生系统临床微生物学与病毒学实验室群 Monitoring the growth and drug susceptibility of individual bacteria using asynchronous magnetic bead rotation sensors b, c, ,
a The University of Michigan, Department of Chemistry, 930 N. University, Ann Arbor, MI 48109-1055, USA
b The University of Michigan, Applied Physics Program, 2477 Randall Laboratory, Ann Arbor, MI 48109-1120, USA
c The University of Michigan, Department of Biomedical Engineering, 2200 Bonisteel, Ann Arbor, MI 48109-2099, USA
d The University of Michigan, Department of Chemical Engineering, 2300 Hayward St., 3074 Dow, Ann Arbor, MI 48109-2136, USA
e The University of Michigan Health System, Clinical Microbiology and Virology Laboratories, 2F461 University Hospital, Box 5054, USA
f The University of Michigan Medical School, Department of Pathology, 1301 Catherine, Ann Arbor, MI 48109-0054, USA
Received 23 July 2010; revised 5 October 2010; accepted 6 October 2010. Available online 14 October 2010.
Abstract
Continuous growth of individual bacteria has been previously studied by direct observation using optical imaging. However, optical microscopy studies are inherently diffraction limited and limited in the number of individual cells that can be continuously monitored. Here we report on the use of the asynchronous magnetic bead rotation (AMBR) sensor, which is not diffraction limited. The AMBR sensor allows for the measurement of nanoscale growth dynamics of individual bacterial cells, over multiple generations. This torque-based magnetic bead sensor monitors variations in drag caused by the attachment and growth of a single bacterial cell. In this manner, we observed the growth and division of individual Escherichia coli, with 80-nm sensitivity to the cell length. Over the life cycle of a cell, we observed up to a 300% increase in the rotational period of the biosensor due to increased cell volume. In addition, we observed single bacterial cell growth response to antibiotics. This work demonstrates the non-microscopy limited AMBR biosensor for monitoring individual cell growth dynamics, including cell elongation, generation time, lag time, and division, as well as their sensitivity to antibiotics.
Keywords: Magnetic bead; Biosensor; Asynchronous magnetic bead rotation; Rotating magnetic field
Article Outline
1. Introduction
2. Materials and methods
2.1. Theoretical derivation
2.2. Cell culture and attachment methods
2.3. Experimental setup and measurement conditions
2.4. Experimental errors
3. Results and discussion
4. Conclusions
Acknowledgements
Appendix A. Supplementary data
References
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFC-517J25T-6&_user=10&_coverDate=01%2F15%2F2011&_rdoc=1&_fmt=full&_orig=search&_origin=search&_cdi=5223&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_use***10&md5=73801be80a2892152e0c101bd486ed63&searchtype=a#sec0025
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作者:admin@医学,生命科学 2011-01-18 23:22
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