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【medical-news】生物医学突破：实验室培养组织血
ScienceDaily (Jan. 12, 2011) — Researchers from Rice University and Baylor College of Medicine (BCM) have broken one of the major roadblocks on the path to growing transplantable tissue in the lab: They've found a way to grow the blood vessels and capillaries needed to keep tissues alive.
The new research is available online and due to appear in the January issue of the journal Acta Biomaterialia.
"The inability to grow blood-vessel networks -- or vasculature -- in lab-grown tissues is the leading problem in regenerative medicine today," said lead co-author Jennifer West, department chair and the Isabel C. Cameron Professor of Bioengineering at Rice. "If you don't have blood supply, you cannot make a tissue structure that is thicker than a couple hundred microns."
As its base material, a team of researchers led by West and BCM molecular physiologist Mary Dickinson chose polyethylene glycol (PEG), a nontoxic plastic that's widely used in medical devices and food. Building on 10 years of research in West's lab, the scientists modified the PEG to mimic the body's extracellular matrix -- the network of proteins and polysaccharides that make up a substantial portion of most tissues.
West, Dickinson, Rice graduate student Jennifer Saik, Rice undergraduate Emily Watkins and Rice-BCM graduate student Daniel Gould combined the modified PEG with two kinds of cells -- both of which are needed for blood-vessel formation. Using light that locks the PEG polymer strands into a solid gel, they created soft hydrogels that contained living cells and growth factors. After that, they filmed the hydrogels for 72 hours. By tagging each type of cell with a different colored fluorescent marker, the team was able to watch as the cells gradually formed capillaries throughout the soft, plastic gel.
To test these new vascular networks, the team implanted the hydrogels into the corneas of mice, where no natural vasculature exists. After injecting a dye into the mice's bloodstream, the researchers confirmed normal blood flow in the newly grown capillaries.
Another key advance, published by West and graduate student Joseph Hoffmann in November, involved the creation of a new technique called "two-photon lithography," an ultrasensitive way of using light to create intricate three-dimensional patterns within the soft PEG hydrogels. West said the patterning technique allows the engineers to exert a fine level of control over where cells move and grow. In follow-up experiments, also in collaboration with the Dickinson lab at BCM, West and her team plan to use the technique to grow blood vessels in predetermined patterns.
The research was supported by the National Science Foundation and the National Institutes of Health. West's work was conducted in her lab at Rice's BioScience Research Collaborative (BRC). The BRC is an innovative space where scientists and educators from Rice University and other Texas Medical Center institutions work together to perform leading research that benefits human medicine and health.
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Biomedical Breakthrough: Blood Vessels for Lab-Grown Tissues
生物医学突破：实验室培养血管组织。
ScienceDaily (Jan. 12, 2011) — Researchers from Rice University and Baylor College of Medicine (BCM) have broken one of the major roadblocks on the path to growing transplantable tissue in the lab: They've found a way to grow the blood vessels and capillaries needed to keep tissues alive.
每日科学（2011年1月12日）—莱斯大学和贝勒医学院（BCM）的研究人员在实验室已经突破了让移植的组织生长的主要障碍之一，他们已经找到了一种培养血管组织和毛细血管生长的方法来让组织存活。
The new research is available online and due to appear in the January issue of the journal Acta Biomaterialia.
这项新的研究文章在网上和一月份的Acta Biomaterialia杂志上可以得到。
"The inability to grow blood-vessel networks -- or vasculature -- in lab-grown tissues is the leading problem in regenerative medicine today," said lead co-author Jennifer West, department chair and the Isabel C. Cameron Professor of Bioengineering at Rice. "If you don't have blood supply, you cannot make a tissue structure that is thicker than a couple hundred microns."
“在实验室培养组织的今天，血管网或脉管系统无法生长仍然是一个主要的问题。”lead co-author Jennifer West, department chair and the Isabel C. Cameron Professor of Bioengineering at Rice说，“如果没有血供，你就不能使一个组织结构长出二百微米。”
As its base material, a team of researchers led by West and BCM molecular physiologist Mary Dickinson chose polyethylene glycol (PEG), a nontoxic plastic that's widely used in medical devices and food. Building on 10 years of research in West's lab, the scientists modified the PEG to mimic the body's extracellular matrix -- the network of proteins and polysaccharides that make up a substantial portion of most tissues.
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作者:admin@医学,生命科学 2011-01-14 00:45
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