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【Stem Cell】The Immunogenicity of Human Adipose-Derived Ce
KEVIN MCINTOSH,a SANJIN ZVONIC,b SARA GARRETT,a JAMES B. MITCHELL,a Z. ELIZABETH FLOYD,b LORA HAMMILL,a AMY KLOSTER,c YUAN DI HALVORSEN,c,d JENNY P. TING,e ROBERT W. STORMS,f BRIAN GOH,b GAIL KILROY,b XIYING WU,a JEFFREY M. GIMBLEb,
Key Words. Human • Mixed lymphocyte reaction • Immunosuppressive • Immunogenic • Multipotent • Tissue engineering Regenerative medicine • Adipose-derived stem cells
ABSTRACT
Regenerative medical techniques will require an abundant source of human adult stem cells that can be readily available at the point of care. The ability to use unmatched allogeneic stem cells will help achieve this goal. Since adipose tissue represents an untapped reservoir of human cells, we have compared the immunogenic properties of freshly isolated, collagenase- digested human adipose tissue-derived stromal vascular
fraction cells (SVFs) relative to passaged, plastic-adherent adipose- derived stem cells (ASCs). Parallel studies have shown that adherence to plastic and subsequent expansion of human adipose-derived cells selects for a relatively homogeneous cell population based on immunophenotype. Consistent with these findings, the presence of hematopoietic-associated markers (CD11a, CD14, CD45, CD86, and histocompatible locus antigen- DR [HLA-DR]) detected on the heterogeneous SVF cell population decreased upon subsequent passage of the ASCs. In mixed lymphocyte reactions (MLRs), SVFs, and early passage ASCs stimulated proliferation by allogeneic responder T cells.
In contrast, the ASCs beyond passage P1 failed to elicit a response from T cells. Indeed, late passage ASCs actually suppressed the MLR response. Although these results support the feasibility of allogeneic human ASC transplantation, confirmatory in vivo animal studies will be required. 认领此文献 The Immunogenicity of Human Adipose-Derived Cells: Temporal Changes In Vitro
脂肪来源细胞免疫原性的体外变化
Key Words. Human • Mixed lymphocyte reaction • Immunosuppressive • Immunogenic • Multipotent • Tissue engineering Regenerative medicine • Adipose-derived stem cells
关键词：人的，混合淋巴细胞反应，免疫抑制剂，免疫原，多能的，组织工程再生医学，脂肪干细胞
Regenerative medical techniques will require an abundant source of human adult stem cells that can be readily available at the point of care. The ability to use unmatched allogeneic stem cells will help achieve this goal. Since adipose tissue represents an untapped reservoir of human cells, we have compared the immunogenic properties of freshly isolated, collagenase- digested human adipose tissue-derived stromal vascular
fraction cells (SVFs) relative to passaged, plastic-adherent adipose- derived stem cells (ASCs).
再生医学在治疗时需要大量的成体干细胞。应用同种异体的干细胞可以解决这一问题。脂肪组织是未被开发利用的细胞储存库，我们对用胶原蛋白酶消化的人脂肪间充质血管细胞的免疫原性进行了比较，与可黏附的脂肪间充质干细胞的传代细胞具有一定的关系。
Parallel studies have shown that adherence to plastic and subsequent expansion of human adipose-derived cells selects for a relatively homogeneous cell population based on immunophenotype. Consistent with these findings, the presence of hematopoietic-associated markers (CD11a, CD14, CD45, CD86, and histocompatible locus antigen- DR [HLA-DR]) detected on the heterogeneous SVF cell population decreased upon subsequent passage of the ASCs. In mixed lymphocyte reactions (MLRs), SVFs, and early passage ASCs stimulated proliferation by allogeneic responder T cells
平行研究认为：脂肪来原细胞的贴壁特性和扩增能力是建立在免疫表型相对接近的基础上的。与研究结果一致的是：和造血细胞相关的表面标记（CD11a,CD14,CD45,CD86,HLA-DR这些标记也可在异种间充质血管细胞出现）随着传代次数的增加将会减少。在混合淋巴细胞反应中，同种T细胞免疫反应可以促进间充质血管细胞和早期脂肪干细胞的传代细胞的增殖
In contrast, the ASCs beyond passage P1 failed to elicit a response from T cells. Indeed, late passage ASCs actually suppressed the MLR response. Although these results support the feasibility of allogeneic human ASC transplantation, confirmatory in vivo animal studies will be required
与之相反的是，脂肪间充质干细胞只有第一代细胞才可引起T细胞免疫反应。事实上，脂肪间充质干细胞的万代细胞确实抑制混合淋巴细胞免疫反应。尽管这些结果支持人同种脂肪干细胞移植的可行性，但仍需要体内动物实验来进一步证实。 脂肪来源细胞免疫原性的体外变化
关键词：人的，混合淋巴细胞反应，免疫抑制剂，免疫原，多能的，组织工程再生医学，脂肪干细胞
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作者:admin@医学,生命科学 2011-06-28 05:48
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