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【文摘发布】鉴定头颈部鳞癌中具有干细胞性质
Athor: Prince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF, Ailles LE.
Resource: Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):973-8. Epub 2007 Jan 8.
Abstract: Like many epithelial tumors, head and neck squamous cell carcinoma (HNSCC) contains a heterogeneous population of cancer cells. We developed an immunodeficient mouse model to test the tumorigenic potential of different populations of cancer cells derived from primary, unmanipulated human HNSCC samples. We show that a minority population of CD44(+) cancer cells, which typically comprise <10% of the cells in a HNSCC tumor, but not the CD44(-) cancer cells, gave rise to new tumors in vivo. Immunohistochemistry revealed that the CD44(+) cancer cells have a primitive cellular morphology and costain with the basal cell marker Cytokeratin 5/14, whereas the CD44(-) cancer cells resemble differentiated squamous epithelium and express the differentiation marker Involucrin. The tumors that arose from purified CD44(+) cells reproduced the original tumor heterogeneity and could be serially passaged, thus demonstrating the two defining properties of stem cells: ability to self-renew and to differentiate. Furthermore, the tumorigenic CD44(+) cells differentially express the BMI1 gene, at both the RNA and protein levels. By immunohistochemical analysis, the CD44(+) cells in the tumor express high levels of nuclear BMI1, and are arrayed in characteristic tumor microdomains. BMI1 has been demonstrated to play a role in self-renewal in other stem cell types and to be involved in tumorigenesis. Taken together, these data demonstrate that cells within the CD44(+) population of human HNSCC possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation and form unique histological microdomains that may aid in cancer diagnosis.
PMID: 17210912 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 呵呵,太忙了,还是把昨天的译文改好更重要.这次就放弃了.不好意思. 欢迎大家认领啊！
积极踊跃者逃版主可能会有加分的奖励哦！（呵呵，我不能保证哦） 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 对不起啊，我这两天事太多了，没有完成任务，欢迎大家重新认领啊！ 怎么大家近来都很忙呢？
重在参与，有问题大家讨论讨论才能共同进步嘛！
再没有人认领我可要下手了哦！ 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Title: Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma.
鉴定头颈部鳞癌中具有干细胞性质的细胞亚群
Athor: Prince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF, Ailles LE.
作者：Prince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF, Ailles LE.
Resource: Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):973-8. Epub 2007 Jan 8.
出处：Proc Natl Acad Sci U S A.2007年1月16日；104(3):973-8. Epub 2007年1月 8日.
Abstract: Like many epithelial tumors, head and neck squamous cell carcinoma (HNSCC) contains a heterogeneous population of cancer cells. We developed an immunodeficient mouse model to test the tumorigenic potential of different populations of cancer cells derived from primary, unmanipulated human HNSCC samples. We show that a minority population of CD44(+) cancer cells, which typically comprise <10% of the cells in a HNSCC tumor, but not the CD44(-) cancer cells, gave rise to new tumors in vivo.
摘要：许多上皮细胞瘤包含异质性癌细胞群体，头颈鳞状上皮细胞肿瘤（癌）(HNSCC)也不例外。 我们从未经处理的原发性人类HNSCC样本分离出不同的癌细胞群体，并建立免疫缺陷性老鼠模型测试这些癌细胞群体的潜在致肿瘤性。我们发现，诱发在体新肿瘤形成的是少数CD44(+)癌细胞（在一个HNSCC肿瘤中，此类癌细胞通常所占细胞比例<10% ），而非CD44(-)癌细胞。
Immunohistochemistry revealed that the CD44(+) cancer cells have a primitive cellular morphology and costain with the basal cell marker Cytokeratin 5/14, whereas the CD44(-) cancer cells resemble differentiated squamous epithelium and express the differentiation marker Involucrin. The tumors that arose from purified CD44(+) cells reproduced the original tumor heterogeneity and could be serially passaged, thus demonstrating the two defining properties of stem cells: ability to self-renew and to differentiate. Furthermore, the tumorigenic CD44(+) cells differentially express the BMI1 gene, at both the RNA and protein levels.
免疫组织化学结果显示，CD44(+)细胞具有原始细胞的形态，并且表达基底细胞标志分子角蛋白5和14，而表达分化标记物－外皮蛋白的CD44(-)癌细胞则与分化的鳞状上皮相似。起源于纯化CD44(+)细胞的肿瘤能够再生原先肿瘤的异质性，并且能够连续传代， 因此证明干细胞具有两个明确的特性：自我更新能力和分化能力。此外，致肿瘤性CD44(+)细胞还具有在RNA和蛋白质水平上表达BMI1基因的特殊性。
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作者:admin@医学,生命科学 2011-03-14 05:11
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