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【Oncogene——编译】一个用来确认和评估乳房癌发
A network of clinically and functionally relevant genes is involved in the reversion of the tumorigenic phenotype of MDA-MB-231 breast cancer cells after transfer of human chromosome 8.[Article]
来源
Oncogene. 24(5):869-879, January 27, 2005.
Accession Number
00006374-200524050-00013.
Author
Seitz, Susanne *,1; Frege, Renate 1; Jacobsen, Anja 2; Weimer, Jorg 2; Arnold, Wolfgang 3; von Haefen, Clarissa 4; Niederacher, Dieter 5; Schmutzler, Rita 6; Arnold, Norbert 2; Scherneck, Siegfried 1
Institution
(1)Department of Tumor Genetics, Max Delbrueck Center for Molecular Medicine, Robert Roessle Str. 10, 13125 Berlin, Germany; (2)Oncology Laboratory, Gynecology and Obstetrics Clinic, University Hospital Schleswig-Holstein Campus Kiel, Michalisstr. 16, 24105 Kiel, Germany; (3)atugen AG, Wiltbergstr. 50, 13125 Berlin, Germany; (4)Department of Hematology, Oncology and Tumor Immunology, Charite-Campus Berlin-Buch, Humboldt University, Lindenberger Weg 80, 13125 Berlin-Buch, Germany; (5)Department of Gynecology and Obstetrics, University of Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Department of Molecular Gynecology and Oncology, Gynecology and Obstetrics Clinic, Kerpener Str. 34, 50931 Koln, Germany
摘要原文
Several investigations have supposed that tumor suppressor genes might be located on human chromosome 8. We used microcell-mediated transfer of chromosome 8 into MDA-MB-231 breast cancer cells and generated independent hybrids with strongly reduced tumorigenic potential. Loss of the transferred chromosome results in reappearance of the malignant phenotype. Expression analysis identified a set of 109 genes (CT8-ps) differentially expressed in microcell hybrids as compared to the tumorigenic MDA-MB-231 and rerevertant cells. Of these, 44.9% are differentially expressed in human breast tumors. The expression pattern of CT8-ps was associated with prognostic factors such as tumor size and grading as well as loss of heterozygosity at the short arm of chromosome 8. We identified CT8-ps networks suggesting that these genes act cooperatively to cause reversion of tumorigenicity in MDA-MB-231 cells. Our findings provide a conceptual basis and experimental system to identify and evaluate genes and gene networks involved in the development and/or progression of breast cancer.
编译
研究指出肿瘤抑制因子可能位于染色体8中。用染色体8微细胞间接转移进入MDA-MB-231乳房癌细胞，产生的独立杂合具有极大的诱发肿瘤趋势。转入的染色体丢失导致恶性表型重新出现。表达分析证明在微细胞杂合中，109个基因序列（CT8-ps）特异的表达（与致肿瘤的MDA-MB-231和复突变细胞细胞对比）。这些基因中44.9%在人乳房肿瘤中特异地表达。CT8-ps的表达特征具有相关的预兆因素（如瘤大小、程度以及染色体8短臂的杂合丢失）。我们证明的CT8-ps网络说明了这些基因共同起作用引起了MDA-MB-231细胞致肿瘤性的反转。这些发现提供了一个用来确认和评估乳房癌发生和/或进展的基因和基因网络的实验体系。 全文！
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作者:admin@医学,生命科学 2011-04-30 17:14
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