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【Nature】转录组测序的一种新的高吞吐量方法
Abstract
Post-transcriptional gene regulation frequently occurs through elements in mRNA 3' untranslated regions (UTRs). Although crucial roles for 3'UTR-mediated gene regulation have been found in Caenorhabditis elegans, most C. elegans genes have lacked annotated 3'UTRs. Here we describe a high-throughput method for reliable identification of polyadenylated RNA termini, and we apply this method, called poly(A)-position profiling by sequencing (3P-Seq), to determine C. elegans 3'UTRs. Compared to standard methods also recently applied to C. elegans UTRs, 3P-Seq identified 8,580 additional UTRs while excluding thousands of shorter UTR isoforms that do not seem to be authentic. Analysis of this expanded and corrected data set suggested that the high A/U content of C. elegans 3'UTRs facilitated genome compaction, because the elements specifying cleavage and polyadenylation, which are A/U rich, can more readily emerge in A/U-rich regions. Indeed, 30% of the protein-coding genes have mRNAs with alternative, partially overlapping end regions that generate another 10,480 cleavage and polyadenylation sites that had gone largely unnoticed and represent potential evolutionary intermediates of progressive UTR shortening. Moreover, a third of the convergently transcribed genes use palindromic arrangements of bidirectional elements to specify UTRs with convergent overlap, which also contributes to genome compaction by eliminating regions between genes. Although nematode 3'UTRs have median length only one-sixth that of mammalian 3'UTRs, they have twice the density of conserved microRNA sites, in part because additional types of seed-complementary sites are preferentially conserved. These findings reveal the influence of cleavage and polyadenylation on the evolution of genome architecture and provide resources for studying post-transcriptional gene regulation.
原文连接
http://www.nature.com/nature/journal/v469/n7328/full/nature09616.html 电子信息文件类型:DOC/Microsoft Word- 文字版⑤高性能与超高性能纤维增强水泥基材料的动态力学行为、动态效应及本构方程,为科学、... 生物医药肿瘤磁致热疗用纳米发热剂的制备方法 本发明是一种用于肿瘤磁致热疗的功能...www.lyinfo.net.cn/...cxy03.doc-2010-09-07-展建成具有国际先进水平的科学研究基地高吞吐量的测序的 34方法表明,动物和植物有大量的小非编码的RNA,其中很多的功能仍未... 加科学家已在世界上首次完成了甲型”H1N1流1感病毒的基因测序工作.的 解开这种新...www.chinesebaojian.com/...472/-2010-09-17-快照-科学研究基地"[标签:content2]
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作者:admin@医学,生命科学 2011-01-08 00:42
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