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【Fast Breaking Comments】Genotypic diversity within a natu
Article Title: Genotypic diversity within a natural coastal bacterioplankton population
Authors: Thompson, JR;Pacocha, S;Pharino, C;Klepac-Ceraj, V;Hunt, DE;Benoit, J;Sarma-Rupavtarm, R;Distel, DL;Polz, MF
Journal: SCIENCE
Volume: 307
Issue: 5713
Page: 1311-1313
Q： Why do you think your paper is highly cited?
A：This study has been cited often because it provides an important environmental context for the expanding fields of microbial genomics and community ecology by quantifying the magnitude of coexisting genomic diversity within a microbial population. In addition, we hope that this work helps to motivate further studies exploring the emergent properties of populations composed of diverse constituents and the ecological and evolutionary mechanisms that give rise to such diversity.
Q： Does it describe a new discovery, methodology, or synthesis of knowledge?
A：Our observation of extreme genomic diversity coexisting within a population (where every microbe in 1000 individuals of a population has a unique genome that can differ in size by up to 1 Mb or ~20%) helps explain the difficulty of assembling genomes from environmental shotgun libraries. In addition, such extreme genomic diversity within populations warrants caution in the extrapolation of the environmental relevance of microbial model systems based on single strains.
Q： Could you summarize the significance of your paper in layman?s terms?
A：Asexual reproduction in bacteria has been long thought to provide a constraint on the evolution of diversity within microbial populations?this is in contrast to sexual reproduction within populations where children share unique combinations of genetic features derived from each parent. However, this study reveals an unprecedented level of diversity within a microbial population that suggests evolution within bacteria occurs by both sexual and asexual mechanisms.
Moreover, the coexistence of such diversity suggests that the unique combinations of genetic traits in each member of the population do not confer significant competitive advantages within the context of the environment observed.
Q： How did you become involved in this research, and were any problems encountered along the way?
A：Our lab has a long-standing interest in microbial ecology and evolution. We were surprised by the high genomic diversity present in environmental isolates of a Vibrio species obtained as part of a seasonal dynamics study of Vibrio diversity in the temperate New England coastal waters (sampling New England coastal waters during the winter certainly posed a challenge!). We decided to follow up on this observation during the following year and found a consistent trend of high genomic diversity associated with this and other species throughout the sampling season.
Janelle Thompson
Post Doctoral Associate
The Polz Lab
Department of Civil and Environmental Engineering
Massachusetts Institute of Technology (MIT)
Cambridge, MA, USA
Martin Polz, Ph.D.
Principal Investigator
The Polz Lab
Assistant Professor of Civil and Environmental Engineering
Department of Civil and Environmental Engineering
Massachusetts Institute of Technology (MIT)
Cambridge, MA, USA 谢谢兄弟。
现在也在弄siRNA吗？ 感谢纤夫关注，我不弄siRNA，我在组织丁香园的生命科学热点进展翻译！
敬请大家热情参与！ Field: Microbiology 微生物学
Article Title: Genotypic diversity within a natural coastal bacterioplankton population
题目：天然海岸浮游菌群的基因多样性
Authors: Thompson, JR;Pacocha, S;Pharino, C;Klepac-Ceraj, V;Hunt, DE;Benoit, J;Sarma-Rupavtarm, R;Distel, DL;Polz, MF
Journal: SCIENCE
Volume: 307
Issue: 5713
Page: 1311-1313
Q： Why do you think your paper is highly cited?
你觉得为什么你的论文得到了高度引用呢？
A：This study has been cited often because it provides an important environmental context for the expanding fields of microbial genomics and community ecology by quantifying the magnitude of coexisting genomic diversity within a microbial population. In addition, we hope that this work helps to motivate further studies exploring the emergent properties of populations composed of diverse constituents and the ecological and evolutionary mechanisms that give rise to such diversity.
这个研究经常被引用是因为，通过在微生物群体中定量分析共生的染色体组的多样性，这个研究为微生物染色体组和群落生态学的扩增提供了一个重要的环境联系。此外，我们希望这个工作能激起更多的人进一步研究探索种群的新出现的性质，这个种群是由多样性的组成以及产生这种多样性的生态的进化的机制共同构成的。
Q： Does it describe a new discovery, methodology, or synthesis of knowledge?
那它是否描述了一个新的发现，方法学或知识的综合呢？
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作者:admin@医学,生命科学 2011-03-06 17:22
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