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【drug-news】丝裂霉素C和5-氟尿嘧啶预防推板切除
Yu Sun, M.D., Li-Xin Wang, M.D., Lei Wang, M.D., Si-Xin Sun, M.D., Xiao-Jian Cao, M.D., Ph.D., Peng Wang, M.D., and Li Feng, M.D.
Department of Orthopedics, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China
Abbreviations used in this paper: BBB = Basso-Beattie-Bresnahan; MMC = mitomycin C; 5FU = 5-fluorouracil.
Address correspondence to: Xiao-Jian Cao, M.D., Ph.D., Department of Orthopedics, First Affiliated Hospital of Nanjing Medical University, Guangzhou Road 300, Nanjing, Jiangsu Province, China. email: xiaojiancao@gmail.com.
DOI: 10.3171/SPI-07/10/423
Object
The effectiveness of the topical application of mitomycin C (MMC) or 5-fluorouracil (5FU) in preventing peridural adhesion after laminectomy was compared in this study.
Methods
Laminectomies were performed at L-1 in 30 rats. Cotton pads soaked with 0.1 mg/ml MMC, 25 mg/ml 5FU, or 9 mg/ml saline (control) were applied to the operative sites. To evaluate neurological deficits pre- and postoperatively, somatosensory evoked potentials were monitored and the Basso-Beattie-Bresnahan locomotion test was performed. Four weeks postlaminectomy the rats were killed, and peridural scar adhesion was evaluated histologically. The level of hydroxyproline, the area of peridural scar tissue, and the number of fibroblasts were determined. The degree of peridural adhesion was classified according to the Rydell standard.
Results
No obvious adhesion formed in the rats in the MMC group, but severe peridural adhesions were found in those in the 5FU and control groups. The content of hydroxyproline, the area of peridural scar tissue, and the number of fibroblasts in the MMC group were significantly lower than those in the 5FU and control groups.
Conclusions
The topical application of MMC rather than 5FU may be a successful method of preventing post-laminectomy peridural adhesions.
Keywords: laminectomy; mitomycin C; peridural adhesion; peridural fibrosis; rat; 5-fluorouracil.
http://thejns.org/doi/abs/10.3171/SPI-07/10/423?cookieSet=1&journalCode=spi
本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领 A comparison of the effectiveness of mitomycin C and 5-fluorouracil in the prevention of peridural adhesion after laminectomy
丝裂霉素C和5-氟尿嘧啶预防推板切除术后硬膜外粘连效果的比较
Abbreviations used in this paper: BBB = Basso-Beattie-Bresnahan; MMC = mitomycin C; 5FU = 5-fluorouracil.
本文所用缩写：BBB = Basso-Beattie-Bresnahan;MMC = 丝裂霉素C;5FU =5-氟尿嘧啶.
Object
The effectiveness of the topical application of mitomycin C (MMC) or 5-fluorouracil (5FU) in preventing peridural adhesion after laminectomy was compared in this study.
目的：这项研究旨在比较局部应用丝裂霉素C和5-氟尿嘧啶预防推板切除术后硬膜外粘连的效果。
Methods
Laminectomies were performed at L-1 in 30 rats. Cotton pads soaked with 0.1 mg/ml MMC, 25 mg/ml 5FU, or 9 mg/ml saline (control) were applied to the operative sites. To evaluate neurological deficits pre- and postoperatively, somatosensory evoked potentials were monitored and the Basso-Beattie-Bresnahan locomotion test was performed. Four weeks postlaminectomy the rats were killed, and peridural scar adhesion was evaluated histologically. The level of hydroxyproline, the area of peridural scar tissue, and the number of fibroblasts were determined. The degree of peridural adhesion was classified according to the Rydell standard.
方法：30只大鼠都已经行L1椎板切除术。将棉片浸透0.1mg/ml MMC, 25ml/mg5-FU或9mg/ml 生理盐水（作对照）后置于手术部位。监测体感诱发电位并完成BBB运动评分以评价术前及术后神经功能缺损。术后4周处死大鼠，组织学评估硬膜外瘢痕粘连。确定羟脯氨酸水平，硬膜外瘢痕组织的范围以及成纤维细胞数量。根据Rydell标准对硬膜外粘连程度进行分级。
Results
No obvious adhesion formed in the rats in the MMC group, but severe peridural adhesions were found in those in the 5FU and control groups. The content of hydroxyproline, the area of peridural scar tissue, and the number of fibroblasts in the MMC group were significantly lower than those in the 5FU and control groups.
结果：在丝裂霉素C组大鼠中无明显的粘连形成，而在5-氟尿嘧啶及对照组中发现严重的粘连。在丝裂霉素C组中的羟脯氨酸含量，硬膜外瘢痕组织的范围以及成纤维细胞数量要显著低于5-氟尿嘧啶组及对照组。
Conclusions
The topical application of MMC rather than 5FU may be a successful method of preventing post-laminectomy peridural adhesions.
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作者:admin@医学,生命科学 2011-04-19 05:11
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