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【medical-news】妊娠早期接触雌激素对胎儿性别发
To study the effects of gestational exposure to estrogen on early gonadal differentiation, pregnant mice were treated by sc injection of diethylstilbestrol (DES) or vehicle from embryonic day (E) 8.5 to E14.5, and gonads at E11.5, E12.5, and E14.5 were examined. Quantitative real-time RT-PCR and in situ hybridization revealed that mRNA levels of steroidogenic factor 1 (SF-1), a key regulator of gonadal differentiation, and several male gonad-specific genes, including Müllerian-inhibiting substance (MIS), the steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage cytochrome P450 (P450scc) and Cerebellin 1 precursor protein (Cbln 1), were significantly decreased in the DES-treated testis compared with the control testis at E12.5 and/or E14.5. Immunohistochemistry demonstrated that the staining intensities for SF-1 and MIS in Sertoli cells were apparently reduced in the DES-treated testis compared with those of the controls at E12.5 and E14.5. Because MIS, StAR, P450scc, and Cbln 1 are activated under the regulation of SF-1, the down-regulation of these factors may be due to reduced SF-1 expression. Immunohistochemistry for laminin-1 demonstrated that ovigerous cords in the DES-treated ovary were smaller than those in controls at E14.5. Moreover, the number of 5-bromo-2'deoxyuridine-5-monophosphate-labeled cells in the DES-treated testis was significantly reduced at E12.5 and E14.5 compared with controls, and that in the DES-treated ovary remained higher than that in the control ovary at E14.5. The results suggest that exogenous estrogens can alter sex-specific genetic pathways governing early differentiation and cell proliferation of both male and female gonads.
Key words: diethylstilbestrol • mouse gonadal differentiation • quantitative real time RT-PCR
http://endo.endojournals.org/cgi/content/abstract/en.2007-1599v1 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Effects of gestational diethylstilbestrol treatment on male and female gonads during early embryonic development
在妊娠期应用雌激素己烯雌酚(Diethylstilbestrol)治疗对雌性和雄性早期胚胎发育中性腺的影响
To study the effects of gestational exposure to estrogen on early gonadal differentiation, pregnant mice were treated by sc injection of diethylstilbestrol (DES) or vehicle from embryonic day (E) 8.5 to E14.5, and gonads at E11.5, E12.5, and E14.5 were examined.
为了研究妊娠期应用雌激素（Estrogen对早期性腺发育的影响，妊娠小鼠从胚胎期E 8.5 到 E14.5皮下注射己烯雌酚或对照， 分别在E11.5, E12.5, 和 E14.5 检测性腺。
Quantitative real-time RT-PCR and in situ hybridization revealed that mRNA levels of steroidogenic factor 1 (SF-1), a key regulator of gonadal differentiation, and several male gonad-specific genes, including Müllerian-inhibiting substance (MIS), the steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage cytochrome P450 (P450scc) and Cerebellin 1 precursor protein (Cbln 1), were significantly decreased in the DES-treated testis compared with the control testis at E12.5 and/or E14.5.
在 E12.5 和/或 E14.5，用实时定量PCR，和原位杂交发现一个关键的性腺发育的调控因子SF-1,和数个雄性性腺特异性基因，包括MIS，StAR，P450scc，Cbln 1的mRNA水平，与对照组睾丸相比，在DES治疗组是显著下降。
Immunohistochemistry demonstrated that the staining intensities for SF-1 and MIS in Sertoli cells were apparently reduced in the DES-treated testis compared with those of the controls at E12.5 and E14.5. Because MIS, StAR, P450scc, and Cbln 1 are activated under the regulation of SF-1, the down-regulation of these factors may be due to reduced SF-1 expression.
在E12.5 和 E14.5，用免疫组化发现在DES治疗组的睾丸支持细胞（Sertoli cell）中SF-1 和MIS染色强度明显减少。因为 MIS, StAR, P450scc, 和Cbln 1均是被调节因子SF-1激活，这些因子的下调可能是由于SF-1的表达下降。
Immunohistochemistry for laminin-1 demonstrated that ovigerous cords in the DES-treated ovary were smaller than those in controls at E14.5.
在 E14.5，laminin-1 免疫组化检测发现在DES治疗组中卵巢的产卵索与对照组比缩小。
Moreover, the number of 5-bromo-2'deoxyuridine-5-monophosphate-labeled cells in the DES-treated testis was significantly reduced at E12.5 and E14.5 compared with controls, and that in the DES-treated ovary remained higher than that in the control ovary at E14.5.
而且，在E12.5 和 E14.5，与对照组相比，5-溴-2'-脱氧尿嘧啶核苷-5单磷酸酯标记的细胞在DES处理睾丸组明显减少；在 E14.5，DES治疗卵巢组中比对照高。
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作者:admin@医学,生命科学 2010-10-08 05:11
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