主页 > 医药生命 >
【J Neurosci】RhoA能增加轴突分支
Soichiro Ohnami, Mitsuharu Endo, Satoshi Hirai, Naofumi Uesaka, Yumiko Hatanaka, Toshihide Yama***a, and Nobuhiko Yamamoto
(see pages 9117–9121)
The Rho family of small GTPases regulates morphology and movement in many cell types by regulating the actin cytoskeleton. Ohnami et al. report that RhoA contributes to activity-induced branching of horizontally growing axons in the upper layers of the cortex in rats. In organotypic slice cultures, increasing RhoA activity pharmacologically or by introducing (via electroporation) a constitutively active form increased the number of short branches of horizontal axons without affecting the number of long branches. Conversely, an inhibitor of RhoA-kinase (a downstream effector of RhoA) reduced branching. Daily live imaging revealed that constitutively active RhoA altered branch dynamics by increasing both elongation and elimination of axons. These data suggest that RhoA increases branch formation without affecting stabilization. To link RhoA to neuronal activity, the authors blocked activity with sodium-channel and glutamate-receptor blockers. These decreased the amount of active RhoA. Furthermore, introducing constitutively active RhoA increased branching when activity was blocked.
http://www.jneurosci.org/cgi/content/full/28/37/i
阅读本文的人还阅读：
作者:admin@医学,生命科学 2010-11-20 05:11
医学,生命科学网