M.T. Duailibi4, S.E. Duailibi4,C.S. Young2, J.D. Bartlett2,J.P. Vacanti3, and P.C. Yelick2
J Dent Res 83(7):523-528, 2004
The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering
applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissueengineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
摘要：最近关于猪牙胚组织的复杂牙齿结构的组织工程技术提示哺乳动物牙再生的潜能。我们通过从3-7天龄大鼠获得的牙胚细胞进行培养提高了组织工程方法。将细胞接种于可吸收性基质中，并植入成年大鼠体内，12周后，取材，检查分析植入物后发现4天龄大鼠牙胚细胞接种于PGA or PLGA 1小时产生的牙齿结构最可靠。我们结论是牙组织工程在猪及大鼠都是可行的。而且我们生成组织工程牙结构实验提示牙上皮和间充质干细胞可在体外维持至少6天 [标签:content1][标签:content2]
作者:admin@医学,生命科学 2011-01-12 17:14