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【编译】生物工程中的一种新支架材料
原题：Characterization of protein release from photocrosslinkable hyaluronic acid-polyethylene glycol hydrogel tissue engineering scaffolds.
作者：Leach JB, Schmidt CE.
单位：Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
摘要：The goal of this work was to utilize the naturally derived bioactive polymer hyaluronic acid (HA) to create a combination tissue engineering scaffold and protein delivery device. HA is a non-immunogenic, non-adhesive glycosaminoglycan that plays significant roles in several cellular processes, including angiogenesis and the regulation of inflammation. In previous work, we created photopolymerizable glycidyl methacrylate-hyaluronic acid (GMHA) hydrogels that had controlled degradation rates, were cytocompatible, and were able to be modified with peptide moieties. In the present studies, we characterized the release of a model protein, bovine serum album in (BSA), from GMHA and GMHA-polyethylene glycol (PEG) hydrogels. Although BSA could be released rapidly (> 60% within 6 h) from 1% GMHA hydrogels, we found that increasing either the GMHA or the PEG concentrations could lengthen the duration of protein delivery. Preliminary size exclusion chromatography studies indicated that the released BSA was almost entirely in its native monomeric form. Lastly, protein release was extended to several weeks by suspending BSA-poly(lactic-co-glycolic acid) microspheres within the hydrogel bulk. These initial studies indicate that the naturally derived biopolymer HA can be employed to design novel photopolymerizable composites that are suitable for delivering stable proteins from scaffolding in tissue engineering applications.
译文：本项研究的目的是寻找一种新的用于组织工程的支架材料，具有可以调控蛋白（包括各种生长因子）释放的特性，以利于新生组织的成熟。光交连透明质酸聚乙烯乙二醇水凝胶photopolymerizable glycidyl methacrylate-hyaluronic acid (GMHA) hydrogels 支架的蛋白释放特性.使用牛血清氨苯磺胺bovine serum album in (BSA),作为释放蛋白,证明了天然生成的生物多聚物hyaluronic acid (HA)能够用来构成复合支架结构发挥控制蛋白释放的作用。为组织工程的研究提供了新的方法。 [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-03-04 17:11
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