主页 > 医学新闻 >
【Can. J. Physiol. Pharmacol】检测＂细胞自噬＂和判断
Yongqiang Chen, Meghan B. Azad, Spencer B. Gibson:Methods for detecting autophagy and determining autophagy-induced cell death;Can. J. Physiol. Pharmacol. 88(3): 285–295 (2010) | doi:10.1139/Y10-010
Yongqiang Chen, Meghan B. Azad, and Spencer B. Gibson
Abstract: Autophagy is an intracellular lysosomal degradation process, which in the case of macroautophagy, is characterized by the formation of double-membraned autophagosomes. Enhanced under stress conditions, autophagy can function to promote cell survival or cell death depending on the type of cellular stress. Interest in autophagy has increased substantially in the past several years as new research implicates this “self-eating” pathway in cell growth, development, and many human diseases. Various methods have been developed for detecting autophagy; however, the implementation of these methods and the interpretation of the results often vary between studies, and a more standardized approach is required. In this review, we summarize the current methods available for detecting autophagy and for determining its contribution to cell death. Furthermore, we discuss the critical points for the successful application of these methods based on experiences from our laboratory and from other research groups.
Key words: autophagy, autophagic cell death, apoptosis, necrosis, mitotic cell death, cell death. 本人认领.24小时内译出! Yongqiang Chen, Meghan B. Azad, Spencer B. Gibson:Methods for detecting autophagy and determining autophagy-induced cell death;Can. J. Physiol. Pharmacol. 88(3): 285–295 (2010)
检测细胞自噬和确定其诱导细胞死亡的方法
HUABIN 初译稿
-------------------------------------------------------------------------
Abstract:
Autophagy is an intracellular lysosomal degradation process, which in the case of macroautophagy, is characterized by the formation of double-membraned autophagosomes.
Enhanced under stress conditions, autophagy can function to promote cell survival or cell death depending on the type of cellular stress.
摘要：
细胞自噬是一种细胞内溶酶体降解之过程，在“巨自噬（macroautophagy）”情况下，它具有能形成双层膜自噬体之特点。
在增加刺激条件下，细胞自噬可依据细胞刺激类型不同，或促进细胞存活或导致细胞死亡。
Interest in autophagy has increased substantially in the past several years as new research implicates this “self-eating” pathway in cell growth, development, and many human diseases.
Various methods have been developed for detecting autophagy; however, the implementation of these methods and the interpretation of the results often vary between studies, and a more standardized approach is required.
在过去数年中，人们已大幅度地增加了对“细胞自噬”领域之新的研究，其暗示在细胞生长发育和许多人类疾病中，存在这种“自食”通道。
为了检测细胞自噬，人们已开发出各种方法；然而，这些方法的实施及其结果的解释，往往在各个研究之间存在很大的差异，因此必须有一个更加规范的做法。
In this review, we summarize the current methods available for detecting autophagy and for determining its contribution to cell death.
Furthermore, we discuss the critical points for the successful application of these methods based on experiences from our laboratory and from other research groups.
在这篇综述中，我们总结了能用于检测细胞自噬的各种现代方法，以便确定细胞自噬对于细胞死亡的作用。
此外，基于我们的实验室和其他研究小组之经验，我们讨论了成功应用这些方法之一些关键问题。
Key words: autophagy, autophagic cell death, apoptosis, necrosis, mitotic cell death, cell death
关键词:自噬，细胞自噬死亡，细胞凋亡，坏死，有丝分裂细胞死亡，细胞死亡
HUABIN 于2010.8.27 子夜 (初译稿) Yongqiang Chen, Meghan B. Azad, Spencer B. Gibson:Methods for detecting autophagy and determining autophagy-induced cell death;Can. J. Physiol. Pharmacol. 88(3): 285–295 (2010)
检测细胞自噬和确定其诱导细胞死亡的方法
HUABIN 初译稿
-------------------------------------------------------------------------
译文整理如下:
摘要：
细胞自噬是一种细胞内溶酶体降解之过程，在“巨自噬（macroautophagy）”情况下，它具有能形成双层膜自噬体之特点。
在增加刺激条件下，细胞自噬可依据细胞刺激类型不同，或促进细胞存活或导致细胞死亡。
在过去数年中，人们已大幅度地增加了对“细胞自噬”领域之新的研究，其暗示在细胞生长发育和许多人类疾病中，存在这种“自食”通道。
阅读本文的人还阅读：
作者:admin@医学,生命科学 2011-01-18 23:28
医学,生命科学网