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【文摘发布】中和抗体在免疫缺陷病毒感染后控
Author: Yamamoto H, Kawada M, Takeda A, Igarashi H, Matano T.
Sourse: PLoS ONE. 2007 Jun 20;2:e540.
BACKGROUND: Unlike most acute viral infections controlled with the appearance of virus-specific neutralizing antibodies (NAbs), primary HIV infections are not met with such potent and early antibody responses. This brings into question if or how the presence of potent antibodies can contribute to primary HIV control, but protective efficacies of antiviral antibodies in primary HIV infections have remained elusive; and, it has been speculated that even NAb induction could have only a limited suppressive effect on primary HIV replication once infection is established. Here, in an attempt to answer this question, we examined the effect of passive NAb immunization post-infection on primary viral replication in a macaque AIDS model. METHODS AND FINDINGS: The inoculums for passive immunization with simian immunodeficiency virus mac239 (SIVmac239)-specific neutralizing activity were prepared by purifying polyclonal immunoglobulin G from pooled plasma of six SIVmac239-infected rhesus macaques with NAb induction in the chronic phase. Passive immunization of rhesus macaques with the NAbs at day 7 after SIVmac239 challenge resulted in significant reduction of set-point plasma viral loads and preservation of central memory CD4 T lymphocyte counts, despite the limited detection period of the administered NAb responses. Peripheral lymph node dendritic cell (DC)-associated viral RNA loads showed a remarkable peak with the NAb administration, and DCs stimulated in vitro with NAb-preincubated SIV activated virus-specific CD4 T lymphocytes in an Fc-dependent manner, implying antibody-mediated virion uptake by DCs and enhanced T cell priming. CONCLUSIONS: Our results present evidence indicating that potent antibody induction post-infection can result in primary immunodeficiency virus control and suggest direct and indirect contribution of its absence to initial control failure in HIV infections. Although difficulty in achieving requisite neutralizing titers for sterile HIV protection by prophylactic vaccination has been suggested, this study points out a possibility of non-sterile HIV control by prophylactic vaccine-induced, sub-sterile titers of NAbs post-infection, providing a rationale of vaccine-based NAb induction for primary HIV control.
PMID: 17579714 本人已认领该文编译，24小时后若未提交译文，请其他战友自由认领。 Title: Post-infection immunodeficiency virus control by neutralizing antibodies.
中和抗体在免疫缺陷病毒感染后控制病毒复制
Author: Yamamoto H, Kawada M, Takeda A, Igarashi H, Matano T.
Sourse: PLoS ONE. 2007 Jun 20;2:e540.
BACKGROUND: Unlike most acute viral infections controlled with the appearance of virus-specific neutralizing antibodies (NAbs), primary HIV infections are not met with such potent and early antibody responses.
背景：大多数急性病毒感染可因病毒特异性中和抗体(NAbs)的出现而得到控制，而原发性HIV感染则与此不同，它并不出现此类早期强效抗体反应。
This brings into question if or how the presence of potent antibodies can contribute to primary HIV control, but protective efficacies of antiviral antibodies in primary HIV infections have remained elusive; and, it has been speculated that even NAb induction could have only a limited suppressive effect on primary HIV replication once infection is established.
那么强效抗体的出现是否并且如何对原发性HIV感染的起到控制作用就成了一个问题，但是否抗病毒抗体在原发性HIV感染中有保护效应仍然不明，而且人们推测就算Nabs可诱导生成也仅只可以有限的在HIV原发性感染初期抑制该病毒的复制。
Here, in an attempt to answer this question, we examined the effect of passive NAb immunization post-infection on primary viral replication in a macaque AIDS model.
为了尝试回答这个问题，我们采用恒河猴动物模型来检测感染后被动免疫Nab对原发性病毒复制所产生的效应。
METHODS AND FINDINGS: The inoculums for passive immunization with simian immunodeficiency virus mac239 (SIVmac239)-specific neutralizing activity were prepared by purifying polyclonal immunoglobulin G from pooled plasma of six SIVmac239-infected rhesus macaques with NAb induction in the chronic phase.
方法与结果：混合六个的慢性感染SIVmac239型猿免疫缺陷病毒恒河猴的血浆，此时血浆内已生成Nab，提纯多克隆免疫球蛋白G，用来制备具有mac239 (SIVmac239)特异性中和活性的被动免疫接种物。
Passive immunization of rhesus macaques with the NAbs at day 7 after SIVmac239 challenge resulted in significant reduction of set-point plasma viral loads and preservation of central memory CD4 T lymphocyte counts, despite the limited detection period of the administered NAb responses.
恒河猴被SIVmac239病毒攻击后7天给予被动免疫Nabs，结果发现尽管抗体作用的观察时间有限，仍检测到血浆病毒载量设定值明显下降，并且主要记忆性CD4 T细胞量得到保存。Peripheral lymph node dendritic cell (DC)-associated viral RNA loads showed a remarkable peak with the NAb administration, and DCs stimulated in vitro with NAb-preincubated SIV activated virus-specific CD4 T lymphocytes in an Fc-dependent manner, implying antibody-mediated virion uptake by DCs and enhanced T cell priming.
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作者:admin@医学,生命科学 2011-08-28 17:15
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