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【文摘发布】[ J Infect Dis]登革病毒感染的小鼠动
题目：Dengue virus infects macrophages and dendritic cells in a mouse model of infection.
作者：Kyle JL, Beatty PR, Harris E.
摘要：Dengue fever is a mosquitoborne viral illness caused by 4 dengue viruses (DENV-1-4). The cellular tropism of DENV has not been definitively determined, despite its importance for understanding viral pathogenesis and identifying therapeutic targets. To define DENV cellular tropism in a small animal model, 129/Pas mice lacking interferon- alpha / beta and/or - gamma receptors were infected with DENV via a subcutaneous route. During the first week after infection, virus was present in lymph nodes, spleen, bone marrow, and circulating white blood cells. F4/80(+)CD11b(+) macrophages and CD11c(+) dendritic cells were demonstrated to be targets for DENV-2 infection in the spleen by flow cytometry directed to structural and nonstructural DENV proteins and by magnetic bead separation followed by strand-specific reverse-transcriptase polymerase chain reaction. We find that the initial cellular tropism of DENV in mice is similar to that reported in humans, thereby paving the way for investigation of cellular tropism and pathogenesis of DENV in primary and secondary infections.
PMID: 17492597 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 来源：J Infect Dis. 2007 Jun 15;195(12):1808-17.
来源：感染疾病杂志.2007 Jun 15;195(12):1808-17.
题目：Dengue virus infects macrophages and dendritic cells in a mouse model of infection.
题目：小鼠登革热病毒可感染巨噬细胞和树突细胞
作者：Kyle JL, Beatty PR, Harris E.
摘要：Dengue fever is a mosquitoborne viral illness caused by 4 dengue viruses (DENV-1-4). The cellular tropism of DENV has not been definitively determined, despite its importance for understanding viral pathogenesis and identifying therapeutic targets.
摘要：登革热是由蚊传播，由4种登革热病毒(DENV-1-4)引起的病毒性疾病。登革热病毒是否具有细胞趋向性尚未确定，尽管是否具有趋向性对认识病毒致病性和确定治疗靶点具有重要意义。
To define DENV cellular tropism in a small animal model, 129/Pas mice lacking interferon- alpha / beta and/or - gamma receptors were infected with DENV via a subcutaneous route.
为了确定在小动物模型中DENV是否具有细胞趋向性，我们对缺乏干扰素alpha / beta和/或gamma的129/Pas小鼠皮下注射了DENV病毒。
During the first week after infection, virus was present in lymph nodes, spleen, bone marrow, and circulating white blood cells. F4/80(+)CD11b(+) macrophages and CD11c(+) dendritic cells were demonstrated to be targets for DENV-2 infection in the spleen by flow cytometry directed to structural and nonstructural DENV proteins and by magnetic bead separation followed by strand-specific reverse-transcriptase polymerase chain reaction.
注射后第一周，病毒见于淋巴结、脾脏、骨髓和外周血白细胞。经特异针对DENV结构和非结构蛋白的脾脏流式细胞仪检测以及经磁珠分离后的链特异性RT-PCR检测发现，F4/80(+)CD11b(+) 的巨噬细胞和CD11c(+)的树突细胞是DENV-2感染的靶点。
We find that the initial cellular tropism of DENV in mice is similar to that reported in humans, thereby paving the way for investigation of cellular tropism and pathogenesis of DENV in primary and secondary infections.
我们发现小鼠中DENV最初的细胞趋向性与人类中的相关报道相似，这为研究DENV的原发和继发感染的细胞趋向性和治病作用铺平了道路。
编译后：约350字
Kyle JL及其同事对小鼠模型中登革热病毒是否可以感染巨噬细胞和树突细胞进行了研究，结果发表在2007.6的感染疾病杂志。登革热是由蚊传播，由4种登革热病毒(DENV-1-4)引起的病毒性疾病。登革热病毒是否具有细胞趋向性尚未确定，尽管是否具有趋向性对认识病毒致病性和确定治疗靶点具有重要意义。为了确定在小动物模型中DENV是否具有细胞趋向性，研究人员对缺乏干扰素alpha / beta和/或gamma的129/Pas小鼠皮下注射了DENV病毒。注射后第一周，病毒见于淋巴结、脾脏、骨髓和外周血白细胞。经特异针对DENV结构和非结构蛋白的脾脏流式细胞仪检测以及经磁珠分离后的链特异性RT-PCR检测发现，F4/80(+)CD11b(+) 的巨噬细胞和CD11c(+)的树突细胞是DENV-2感染的靶点。研究人员发现小鼠中DENV最初的细胞趋向性与人类中的相关报道相似，这为研究DENV的原发和继发感染的细胞趋向性和治病作用铺平了道路。 [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-05-30 19:24
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