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【文摘发布】口服预防癌症的黑覆盆子冷冻干燥
Author：Susan R. Mallery1 , Gary D. Stoner3, 5, Peter E. Larsen1, Henry W. Fields2, Kapila A. Rodrigo1, Steven J. Schwartz4, Qingguo Tian4, Jin Dai6 and Russell J. Mumper6
Resource:Pharmaceutical Research, 24(4),728-737
Abstract：
Purpose The purpose of these studies was to formulate mucoadhesive gels containing freeze dried black raspberries (FBR) and to determine optimum parameters for a subset of FBR bioactive compounds including anthocyanin stability, absorption and penetration in-vitro and in-vivo.
Materials and Methods Berry gels were prepared having FBR at 5% and 10% w/w and final pHs ranging from 3.5 to 7.5. A HPLC assay was developed to quantify and determine the stability of the anthocyanins in the gels. A single time-point study was performed to determine anthocyanin uptake when the gels were applied to oral mucosa. Penetration of anthocyanins into human oral tissue explants was determined as a function of gel pH and FBR content. A HPLC-mass spectroscopy assay was utilized to quantify the anthocyanin levels in human oral tissue explants, saliva, and blood.
Results The stability of anthocyanins in the gel was directly related to gel pH and storage temperature. Maximum stability of anthocyanins was found at lower pH (pH 3.5) and storage temperature (4°C). Anthocyanins contained in mucoadhesive berry gel formulations were readily absorbed into human oral mucosa tissue as evidenced by detectable blood levels within 5 min after gel application. There was a trend for greater penetration of anthocyanins into tissue explants for berry gels with a final pH of 6.5 versus pH 3.5.
Conclusions Formulation and characterization of a novel gel formulation for local delivery of chemopreventive compounds to human oral mucosal tissues has been described. The results show anthocyanin stability was dependent upon gel pH and storage temperature and also demonstrate that the gel composition is well-suited for absorption and penetration into the target oral mucosal tissue site.
PMID:17372698
【文摘发布】文摘类新闻投稿格式专贴
http://www.dxy.cn/bbs/post/view?bid=116&id=8600034&sty=1&tpg=1&age=0 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Title：Formulation and In-Vitro and In-Vivo Evaluation of a Mucoadhesive Gel Containing Freeze Dried Black Raspberries: Implications for Oral Cancer Chemoprevention
标题：口服预防癌症的冷冻干燥黑覆盆子凝胶粘膜粘附制剂的处方研究及体内外评价
Author：Susan R. Mallery1 , Gary D. Stoner3, 5, Peter E. Larsen1, Henry W. Fields2, Kapila A. Rodrigo1, Steven J. Schwartz4, Qingguo Tian4, Jin Dai6 and Russell J. Mumper6
作者：Susan R. Mallery1 , Gary D. Stoner3, 5, Peter E. Larsen1, Henry W. Fields2, Kapila A. Rodrigo1, Steven J. Schwartz4, Qingguo Tian4, Jin Dai6 and Russell J. Mumper6
Resource: Pharmaceutical Research, 24(4),728-737
文章来源：药学研究，24(4),728-737
Abstract：
摘要：
Purpose The purpose of these studies was to formulate mucoadhesive gels containing freeze dried black raspberries (FBR) and to determine optimum parameters for a subset of FBR bioactive compounds including anthocyanin stability, absorption and penetration in-vitro and in-vivo.
目的：研究目的是制备冷冻干燥黑覆盆子（FBR）凝胶粘膜粘附剂并确定一组FBR生物活性化合物的最佳参数，包括花青素在体内外的稳定性、吸收和渗透性。
Materials and Methods Berry gels were prepared having FBR at 5% and 10% w/w and final pHs ranging from 3.5 to 7.5. A HPLC assay was developed to quantify and determine the stability of the anthocyanins in the gels. A single time-point study was performed to determine anthocyanin uptake when the gels were applied to oral mucosa. Penetration of anthocyanins into human oral tissue explants was determined as a function of gel pH and FBR content. A HPLC-mass spectroscopy assay was utilized to quantify the anthocyanin levels in human oral tissue explants, saliva, and blood.
材料和方法：制备含5%和10% w/w FBR的浆果凝胶，并且最终pH值控制在3.5 到 7.5。用HPLC定量并确定凝胶中花青素的稳定性。进行单时间点研究来确定凝胶应用于口腔粘膜时花青素的吸收。花青素在人口腔组织外植体中的渗透性由凝胶的pH值和FBR容量决定。用HPLC-质谱分析对人口腔粘膜组织外植体、唾液和血液中的花青素水平进行定量。
Results The stability of anthocyanins in the gel was directly related to gel pH and storage temperature. Maximum stability of anthocyanins was found at lower pH (pH 3.5) and storage temperature (4°C). Anthocyanins contained in mucoadhesive berry gel formulations were readily absorbed into human oral mucosa tissue as evidenced by detectable blood levels within 5 min after gel application. There was a trend for greater penetration of anthocyanins into tissue explants for berry gels with a final pH of 6.5 versus pH 3.5.
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作者:admin@医学,生命科学 2010-11-29 05:11
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