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【bio-news】活体RNA染色剂（RNA dyes for life）
Commercially available RNA stains work well on fixed cells, but have had mixed results in live applications. Young-Tae Chang at New York University and colleagues may have developed novel dyes that work better in vivo.
目前已商业化的那些RNA染色剂能够很好的使用于已固定细胞，但是应用于活体时，则效果参差不齐，不尽人意。New York University 的 Young-Tae Chang和他的同事们也许已经发明了一种新的能更好地适用于活体的RNA染色剂。
Chang screened a library of styryl dyes for response to RNA in solution, high signal intensity, and low photobleaching. He selected three candidate dyes for testing in four different cell types. Comparing the results of his novel dyes with that of RNA Select and SYTO, the commercially available dyes manufactured by Invitrogen, his group found that SYTO worked only in fixed-cell applications. "We screened it for many conditions but could not make it work in live cells," says Chang, who patented the dyes through NYU and is hoping to commercialize them soon.
Chang 将各种苯乙烯基染料对溶液中的RNA的反应，按高信号强度和低光致退色的特性进行了筛选。他将三种候选的染料对四种不同的细胞进行染色实验。他还将其发现的获选RNA染色剂与Invitrogen 出品的一种已商业化了染色剂 SYTO 进行了比较。他们发现SYTO仅能应用于已固定细胞。“我们在不同的条件下进行了实验，但SYTO还是不能应用于活体细胞”，Chang说。 他们已将此染色剂通过NYU 申请了专利，希望不久的将来能看到其商业化的产品。
Jason Kilgore, Cell Biologist for Invitrogen, maintains that the SYTO labeling patterns are comparable in fixed and live cells. But it is possible that the different cell types could be responsible for the results. Stephen Yue, who developed the SYTO dye for Invitrogen, was skeptical about chemical structure of Chang's new dye, "Styryl dyes stick to a lot of cellular compartments, especially at the membrane, causing a general staining of the whole cell."
Invitrogen的细胞生物学家Jason Kilgore 辩解说， SYTO染色剂既适用于已固定的细胞，也完全可用于活体细胞的标记。 也许是不同的细胞类型导致了Chang的实验结果。 Stephen Yue，Invitrogen 产品SYTO的发明者，也置疑 Chang 的新染色剂的化学结构，“苯乙烯基染料对许多细胞结构都有亲和力，尤其是膜类结构，从而导致整个细胞的全面受染。”
To inquire about the reagent, contact Chang at yt.chang@nyu.edu.
1. Chang YT, et al., "RNA-selective, live cell imaging probes for studying nuclear structure and function," Chem Biol, 13:615-23, 2006. [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-02-13 05:11
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