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【文摘发布】凝胶电泳与电感耦合等离子质谱联
Author：Wolfram Brüchert, Andreas Helfrich, Nico Zinn, Thomas Klimach, Markus Breckheimer, Hongwei Chen, Senchao Lai, Thorsten Hoffmann, and Jörg Bettmer
Resource: Analytical Chemistry, 2007,79 (4) ：1714 -1719.
Abstract：In this paper, we present an online coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICP-MS) for the determination of iodine species (iodide and iodate) in liquid (seawater) and aerosol samples. For the first time, this approach is applied to the analysis of small molecules, and initial systematic investigations revealed that the migration behavior as well as the detection sensitivity strongly depends on the matrix (e.g., high concentrations of chloride). These effects could consequently affect the accuracy of analytical results, so that they need to be considered for the analysis of real samples. The technique used for quantification is species-specific isotope dilution analysis (ssIDA), which is a matrix-independent calibration method under certain conditions. We demonstrate that the use of 129I-enriched iodide and iodate allows the correction of the impact of the matrix on both, the electrophoretic migration and the detection sensitivity of the ICP-MS. After optimization, this coupling offers a novel and alternative method in the analysis of iodine compounds in various matrices. Here, we demonstrate the analytical capability of the technique for the chemical characterization of marine aerosols. The results show the presence of iodide and iodate at the ng m-3 and sub-ng m-3 level in the investigated aerosol samples, which were taken at the coastal research station in Mace Head, Ireland. These results are in good agreement with other recent studies, which demonstrated that the iodine chemistry in the marine atmosphere is only poorly understood. In addition to iodide and iodate, another iodine compound could be separated and detected in certain samples with high total iodine concentrations and was identified as elemental iodine, probably in form of triiodide, by peak matching. However, it may arise from an artifact during sample preparation.
PMID: 17297978 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Title：Gel Electrophoresis Coupled to Inductively Coupled Plasma-Mass Spectrometry Using Species-Specific Isotope Dilution for Iodide and Iodate Determination in Aerosols
标题：凝胶电泳与电感耦合等离子质谱联用（特异性同位素稀释法）测定气溶胶中碘化物和碘酸盐
Author：Wolfram Brüchert, Andreas Helfrich, Nico Zinn, Thomas Klimach, Markus Breckheimer, Hongwei Chen, Senchao Lai, Thorsten Hoffmann, and Jörg Bettmer
作者：Wolfram Brüchert, Andreas Helfrich, Nico Zinn, Thomas Klimach, Markus Breckheimer, Hongwei Chen, Senchao Lai, Thorsten Hoffmann, and Jörg Bettmer
Resource: Analytical Chemistry, 2007,79 (4) ：1714 -1719.
文章来源：分析化学, 2007,79 (4) ：1714 -1719
Abstract：In this paper, we present an online coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICP-MS) for the determination of iodine species (iodide and iodate) in liquid (seawater) and aerosol samples.
摘要：
在这篇论文中，我们提供一项在线联合凝胶电泳（GE）和电感耦合等离子体质谱 (ICP-MS)测定液体（海水）和气溶胶样品中的碘类物质（碘化物和碘酸盐）的方法。
For the first time, this approach is applied to the analysis of small molecules, and initial systematic investigations revealed that the migration behavior as well as the detection sensitivity strongly depends on the matrix (e.g., high concentrations of chloride).
这种技术第一次用于分析小分子，而最初的系统调查研究显示迁移行为和检测灵敏度都强烈的依赖基质（如高浓度的氯化物）。
These effects could consequently affect the accuracy of analytical results, so that they need to be considered for the analysis of real samples.
因为这些效应会影响分析结果的精确度，因此需要考虑对实际样品进行分析。
The technique used for quantification is species-specific isotope dilution analysis (ssIDA), which is a matrix-independent calibration method under certain conditions.
这种定量分析技术称为特异性同位素稀释法分析(ssIDA)，是一种在特定条件下不依赖基质的校准方法。
We demonstrate that the use of 129I-enriched iodide and iodate allows the correction of the impact of the matrix on both, the electrophoretic migration and the detection sensitivity of the ICP-MS.
我们证实使用富含129I的碘化物和碘酸盐可以校正ICP-MS中电泳迁移和检测灵敏度的基质影响。
After optimization, this coupling offers a novel and alternative method in the analysis of iodine compounds in various matrices.
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作者:admin@医学,生命科学 2010-10-29 05:11
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