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【文摘发布】<Cell>高效能性骨骼肌干细胞移
Highly Efficient, Functional Engraftment of Skeletal Muscle Stem Cells in Dystrophic Muscles
Massimiliano Cerletti,1,3 Sara Jurga,1,3 Carol A. Witczak,2 Michael F. Hirshman,2 Jennifer L. Shadrach,1,3 Laurie J. Goodyear,2 and Amy J. Wagers1,3,
Summary
Satellite cells reside beneath the basal lamina of skeletal muscle fibers and include cells that act as precursors for muscle growth and repair. Although they share a common anatomical localization and typically are considered a homogeneous population, satellite cells actually exhibit substantial heterogeneity. We used cell-surface marker expression to purify from the satellite cell pool a distinct population of skeletal muscle precursors (SMPs) that function as muscle stem cells. When engrafted into muscle of dystrophin-deficient mdx mice, purified SMPs contributed to up to 94% of myofibers, restoring dystrophin expression and significantly improving muscle histology and contractile function. Transplanted SMPs also entered the satellite cell compartment, renewing the endogenous stem cell pool and participating in subsequent rounds of injury repair. Together, these studies indicate the presence in adult skeletal muscle of prospectively isolatable muscle-forming stem cells and directly demonstrate the efficacy of myogenic stem cell transplant for treating muscle degenerative disease 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Highly Efficient, Functional Engraftment of Skeletal Muscle Stem Cells in Dystrophic Muscles
在肌营养不良肌肉中进行的高度有效的骨骼肌干细胞的功能移植
Massimiliano Cerletti,1,3 Sara Jurga,1,3 Carol A. Witczak,2 Michael F. Hirshman,2 Jennifer L. Shadrach,1,3 Laurie J. Goodyear,2 and Amy J. Wagers1,3,
Summary
概要
Satellite cells reside beneath the basal lamina of skeletal muscle fibers and include cells that act as precursors for muscle growth and repair.
肌卫星细胞位于骨骼肌的基底层下，也包括供肌肉生长和修复的前体细胞。
Although they share a common anatomical localization and typically are considered a homogeneous population, satellite cells actually exhibit substantial heterogeneity.
尽管它们在解剖位置上相同并且被典型的认为是一个同质群，但是它们实际上还是表现出本质上的异质性。
We used cell-surface marker expression to purify from the satellite cell pool a distinct population of skeletal muscle precursors (SMPs) that function as muscle stem cells.
我们用细胞表面标记的表达去纯化卫星细胞，从不同种类的骨骼肌前体细胞所含有的卫星细胞库里，前体细胞行使着肌肉干细胞的功能。
When engrafted into muscle of dystrophin-deficient mdx mice, purified SMPs contributed to up to 94% of myofibers, restoring dystrophin expression and significantly improving muscle histology and contractile function. Transplanted SMPs also entered the satellite cell compartment, renewing the endogenous stem cell pool and participating in subsequent rounds of injury repair.
当这些纯化的细胞移植进DDM小鼠时，它们标记了94%的肌纤维，重建了营养不良基因的表达，并且改善了肌肉的组织和收缩功能。移植入的骨骼肌前体细胞也进入了卫星细胞间隔，更新了内源性的干细胞库和参与了后来的损伤修复。
Together, these studies indicate the presence in adult skeletal muscle of prospectively isolatable muscle-forming stem cells and directly demonstrate the efficacy of myogenic stem cell transplant for treating muscle degenerative disease
总之，这些研究提示在成年人骨骼肌里存在可分离的将形成肌肉的干细胞，并且直接证明了在治疗肌肉退行性疾病方面肌源性干细胞移植的有效性。
编译：
在肌营养不良肌肉中进行的高度有效的骨骼肌干细胞的功能移植
概要
肌卫星细胞位于骨骼肌的基底层下，也包括供肌肉生长和修复的前体细胞。尽管它们在解剖位置上相同并且被典型的认为是一个同质群，但是它们实际上还是表现出本质上的差别。
我们用细胞表面标记的表达去纯化这些卫星细胞，从不同种类的骨骼肌前体细胞所含有的卫星细胞库里—骨骼肌前体细胞行使着肌肉干细胞的功能。当这些纯化的细胞移植进DDM小鼠时，它们参与了94%的肌纤维的产生，重启了营养不良基因的表达，并且改善了肌肉的组织和收缩功能。移植入的骨骼肌前体细胞也进入了卫星细胞间隔，更新内源性的干细胞库和参与了后来的损伤修复。总之，这些研究提示在成年人骨骼肌里存在可分离的将形成肌肉的干细胞，并且直接证明了在治疗肌肉退行性疾病方面肌源性干细胞移植的有效性。 供参考
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作者:admin@医学,生命科学 2011-04-06 17:11
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