Tissue-Engineered Skin Containing Mesenchymal Stem Cells Improves Burn Wounds
*†‡Peng Liu, §Zhihong Deng, ‡Shufang Han, *†Tao Liu, ¶Ning Wen, *†Wei Lu, †Xianhui Geng, *†Sha Huang, and *†Yan Jin
*Department of Oral Histology and Pathology, School of Stomatology; †Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi; ‡General Hospital of Jinan Military Region, PLA, Jinan, Shandong; §Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi; and ¶Department of Stomatology, Beijing 301 General Hospital of PLA, Beijing, China
Correspondence to Prof. Yan Jin, Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. E-mail: firstname.lastname@example.org
Copyright Journal compilation © 2008 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Animal model • Mesenchymal stem cells • Wound healing • Tissue engineering
There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue-engineered (TE) skin combined with bone MSCs, we established a burn wound model in the porcine skin. With a controlling temperature and time of the burning device to obtain different degrees of burn wounds, a deep dermal partial thickness burn was introduced to the porcine skin using a heated-brass contact injury at 100°C for 20 s. Collagen-GAG scaffolds were utilized as the matrix; MSCs separated from pigs were seeded on them to form the skin equivalent. When grafted to the burn wounds, the TE skin containing MSCs showed better healing and keratinization, less wound contraction, and more vascularization. Grafts proliferated well and contributed to the neo-tissues. These data suggest that TE skin containing MSCs in a burn defect can accelerate wound healing and receive satisfactory effects. 美国《每日科学》报道
Bone Marrow Stem Cells Used To Regenerate Skin
ScienceDaily (Jan. 19, 2009) — A new study suggests that adult bone marrow stem cells can be used in the construction of artificial skin. The findings mark an advancement in wound healing and may be used to pioneer a method of organ reconstruction. The study is published in Artificial Organs.*
To investigate the practicability of repairing burn wounds with tissue-engineered skin combined with bone marrow stem cells, the study established a burn wound model in the skin of pigs, which is known to be anatomically and physiologically similar to human skin.
Engineering technology and biomedical theory methods were used to make artificial skin with natural materials and bone marrow derived stem cells. Once the artificial skin was attached to the patient and the dermal layer had begun to regenerate, stem cells were differentiated into skin cells. The cells are self-renewing and raise the quality of healing in wound healing therapy. When grafted to the burn wounds, the engineered skin containing stem cells showed better healing, less wound contraction and better development of blood vessels.
作者:admin@医学,生命科学 2011-02-13 17:12