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【Nature AOP编译】研究证实调整内吞途经的激酶比
Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis
来源
Nature advance online publication; published online 11 May 2005 | 10.1038/nature03571
http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature03571.html
摘要原文
Endocytosis is a key cellular process, encompassing different entry routes and endocytic compartments. To what extent endocytosis is subjected to high-order regulation by the cellular signalling machinery remains unclear. Using high-throughput RNA interference and automated image analysis, we explored the function of human kinases in two principal types of endocytosis: clathrin- and caveolae/raft-mediated endocytosis. We monitored this through infection of vesicular stomatitis virus, simian virus 40 and transferrin trafficking, and also through cell proliferation and apoptosis assays. Here we show that a high number of kinases are involved in endocytosis, and that each endocytic route is regulated by a specific kinase subset. Notably, one group of kinases exerted opposite effects on the two endocytic routes, suggesting coordinate regulation. Our analysis demonstrates that signalling functions such as those controlling cell adhesion, growth and proliferation, are built into the machinery of endocytosis to a much higher degree than previously recognized.
编译
内吞作用是一个主要的细胞调节过程，包含了不同的进入途径和多种的胞吞位置。内吞作用多大程度地由细胞信号机制调节还不清楚。利用高流通量RNA干扰和自动影响分析，作者研究了人类激酶在两种主要类型的内吞作用—笼形蛋白和胞膜窖介导的内吞作用。作者通过感染水泡性口膜炎病毒和SV40病毒以及专铁蛋白运输，还通过细胞增殖和凋亡分析来监测激酶的功能。我们证明大量的激酶在内吞作用中起作用，并且每种内吞途径都由特定激酶亚型调节。值得注意的是，一些激酶在这两种内吞途径中起了相反的作用，说明了协同调节作用。这些分析说明，象控制细胞黏着、生长、增殖这样的信号发放功能在内吞作用机制中起了比先前认识到的还要强的功能。 [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-03-30 15:22
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