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【medical-news】在大鼠脊髓植入的人类干细胞可分
Public release date: 13-Feb-2007
Human stem cell transplants mature into neurons and make contacts in rat spinal cord
Human nerve stem cells transplanted into rats' damaged spinal cords have survived, grown and in some cases connected with the rats' own spinal cord cells in a Johns Hopkins laboratory, overturning the long-held notion that spinal cords won't allow nerve repair.
A report on the experiments will be published online this week at PLoS Medicine and "establishes a new doctrine for regenerative neuroscience," says Vassilis Koliatsos, M.D., associate professor of neuropathology at Johns Hopkins. "The spinal cord, a part of the nervous system that is thought of as incapable of repairing itself, can support the development of transplanted cells," he added.
"We don't yet know whether the connections we've seen can transmit nerve signals to the degree that a rat could be made to walk again," says Koliatsos, "We're still in the proof of concept stage, but we're making progress and we're encouraged."
In their experiments, the scientists gave anesthetized rats a range of spinal cord injuries to lesion or kill motor neurons or performed sham surgeries. They varied experimental conditions to see if the presence or absence of spinal cord lesions had an effect on the survival and maturation of human stem cell grafts. Two weeks after lesion or sham surgery, they injected human neural stem cells into the left side of each rat's spinal cord.
After six months, the team found more than three times the number of human cells than they injected in the damaged cords, meaning the transplanted cells not only survived but divided at least twice to form more cells. Moreover, says Koliatsos, the cells not only grew in the area around the original injection, but also migrated over a much larger spinal cord territory.
Three months after injection, the researchers found evidence that some of the transplanted cells developed into support cells rather than nerve cells, while the majority became mature nerve cells. High-powered microscopic examination showed that these nerve cells appear to have made contacts with the rat's own spinal cord cells. 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 期待中!!! Human stem cell transplants mature into neurons and make contacts in rat spinal cord
植入脊髓的人类干细胞可分化为成熟的神经元并形成突触
Human nerve stem cells transplanted into rats' damaged spinal cords have survived, grown and in some cases connected with the rats' own spinal cord cells in a Johns Hopkins laboratory, overturning the long-held notion that spinal cords won't allow nerve repair.
在Johns Hopkins实验室植入被破坏的大鼠脊髓的人类干细胞可以生存、成长，在一些情况下与大鼠体内的神经神经细胞相互联系，这推翻了长期以来的一种观点：脊髓不能修复。
A report on the experiments will be published online this week at and "establishes a new doctrine for regenerative neuroscience," says Vassilis Koliatsos, M.D., associate professor of neuropathology at Johns Hopkins. "The spinal cord, a part of the nervous system that is thought of as incapable of repairing itself, can support the development of transplanted cells," he added.
该实验的一篇报道将在本周《PLoS Medicine》网络版报道，“这建立了一条新的再生神经科学理论，”Johns Hopkins 神经病理学副教授医学博士Vassilis Koliatsos补充说，“脊髓，作为神经系统的一部分，一向被认为不能修复自身，推动了细胞移植的研究。”
"We don't yet know whether the connections we've seen can transmit nerve signals to the degree that a rat could be made to walk again," says Koliatsos, "We're still in the proof of concept stage, but we're making progress and we're encouraged."
“我们现在还不知道现在观察到的联系是否能传递神经信号足以能使大鼠活动，Koliatsos说，”目前我们仍处于寻找推测结论的证据阶段，但是我们仍获得了一定的进展，由此我们倍受鼓励。”
In their experiments, the scientists gave anesthetized rats a range of spinal cord injuries to lesion or kill motor neurons or performed sham surgeries. They varied experimental conditions to see if the presence or absence of spinal cord lesions had an effect on the survival and maturation of human stem cell grafts. Two weeks after lesion or sham surgery, they injected human neural stem cells into the left side of each rat's spinal cord.
在他们实验中科学家使麻醉的大鼠脊髓受到不同程度的伤害：使神经受损伤或者破坏神经元或者实施脊髓损害手术。他们摸索了各种实验条件以观察脊髓损伤或者不损伤的的条件下是否对移植的人类干细胞的生存或者成熟有影响。脊髓损伤或者实施脊髓损害手术后两周，他们将人类神经干细胞移植到每只大鼠脊髓的左侧。
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作者:admin@医学,生命科学 2011-07-22 18:41
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