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【文摘发布】人鼻咽癌细胞系中肿瘤干细胞样S
Title: Identification of Cancer Stem Cell-Like Side Population Cells in Human Nasopharyngeal Carcinoma Cell Line.
题目：人鼻咽癌细胞系中肿瘤干细胞样SP细胞的鉴定
Resource: Cancer Res. 2007 Apr 15; 67:3716-3724
2005 IF: 7.6
Abstract: Side population (SP) cells have been isolated from several solid tumors. They lack distinct molecular markers for cancer stem cells (CSC) and increasing evidence suggests that they may play an important role in tumorigenesis and cancer therapy. However, there are no reports about the existence and function of SP cells in nasopharyngeal carcinoma (NPC) cells thus far. In this study, we scanned SP cells from five NPC cell lines and investigated stem cell characteristics, such as proliferation, self-renewal, and differentiation, using SP cells from the widely-used CNE-2 NPC cell line. We observed a strong tumorigenesis ability of SP cells following in vivo transplantation into nonobese diabetic/severe combined immunodeficient mice. Immunofluorescence revealed that cytokine 19 was highly expressed on SP cells. SP cells were found to be more resistant to chemotherapy and radiotherapy and this was related to the ATP-binding cassette half transporter member 2 of G family protein and Smoothened protein expression, respectively. Our results not only showed that SP cells in human NPC cell line CNE-2 had stem cell characteristics in vitro but also showed that they had a strong ability to form tumors in vivo. Importantly, we found the cell marker, cytokine 19, may serve as a potential molecular marker for further characterization of CSC. Taken together, our data shed light on tumorigenesis and therapeutic-resistant mechanisms, which are helpful for developing novel targets for effective clinical treatment of NPC.
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谢谢Zhangxm43的支持！ Author: Wang J, Guo LP, Chen LZ, Zeng YX, Lu SH
作者：Wang J, Guo LP, Chen LZ, Zeng YX, Lu SH
Title: Identification of Cancer Stem Cell-Like Side Population Cells in Human Nasopharyngeal Carcinoma Cell Line.
题目：人鼻咽癌细胞系中肿瘤干细胞样SP细胞的鉴定
Resource: Cancer Res. 2007 Apr 15; 67 :3716-3724
发表期刊：Cancer Res（2005年影响因子：7.6）. 2007 Apr 15; 67 :3716-3724
Abstract: Side population (SP) cells have been isolated from several solid tumors. They lack distinct molecular markers for cancer stem cells (CSC) and increasing evidence suggests that they may play an important role in tumorigenesis and cancer therapy. However, there are no reports about the existence and function of SP cells in nasopharyngeal carcinoma (NPC) thus far. In this study, we scanned SP cells from five NPC cell lines and investigated stem cell characteristics, such as proliferation, self-renewal, and differentiation, using SP cells from the widely-used CNE-2 NPC cell line. We observed a strong tumorigenesis ability of SP cells following in vivo transplantation into nonobese diabetic/severe combined immunodeficient mice. Immunofluorescence revealed that cytokine 19 was highly expressed on SP cells. SP cells were found to be more resistant to chemotherapy and radiotherapy and this was related to the ATP-binding cassette half transporter member 2 of G family protein and Smoothened protein expression, respectively. Our results not only showed that SP cells in human NPC cell line CNE-2 had stem cell characteristics in vitro but also showed that they had a strong ability to form tumors in vivo. Importantly, we found the cell marker, cytokine 19, may serve as a potential molecular marker for further characterization of CSC. Taken together, our data shed light on tumorigenesis and therapeutic-resistant mechanisms, which are helpful for developing novel targets for effective clinical treatment of NPC.
摘要：干细胞样的侧群细胞（SP）已经在几种实体瘤中被分离提取。它们缺少肿瘤干细胞（CSC）的明显的分子标志，但是越来越多的证据显示它们可能在肿瘤的发生和肿瘤治疗中有很重要的作用。但是迄今为止尚无关于鼻咽癌中SP细胞存在及其功能的研究。在这项研究中，我们从5个鼻咽癌细胞系得到SP细胞，并用经常使用的CNE-2鼻咽癌细胞系得到的SP细胞进行了其干细胞特性的研究，如：细胞的增殖、自我更新和分化等。我们发现SP细胞在体内移植的重度联合免疫缺陷鼠中具有很强的致瘤能力。免疫荧光显示SP细胞中细胞因子19的高表达。SP细胞对放、化疗具有抗性的原因可能各自与ATP结合盒转运子G2（ABCG2）和smo蛋白的表达有关。我们的研究结果不仅在体外证实人鼻咽癌CNE-2细胞系中存在肿瘤干细胞样的SP细胞，而且在体内模型中具有很强的成瘤作用。更重要的是，我们发现细胞因子19可能是鼻咽癌肿瘤干细胞的一个特异分子标记。总之，我们的数据显示了鼻咽癌SP细胞的成瘤和放化疗耐受的机制，这些都为临床上有效治疗鼻咽癌提供了新型靶标。 关于SP细胞的一篇综述见下：
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作者:admin@医学,生命科学 2011-06-23 05:17
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