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【文摘发布】移植肾的Tim-3表达
Authoronciano VC, Renesto PG, Nogueira E, Rangel EB, Cenedeze MA, Franco MF, Camara NO, Pacheco-Silva A.
Source: Transpl Immunology. 2007 Apr;17(3):215-22
Abstract：BACKGROUND: Tim-3 was recently described as a Th1-specific molecule, participating in the regulation of immune responses and in the induction of allograft tolerance. Here, we studied Tim-3 mRNA expression together with molecular markers of T-cell activation and cytotoxicity, in rejected human kidney grafts. METHODS: Twenty human kidney grafts that had undergone nephrectomy due to an irreversible acute rejection episode were studied. We quantified intragraft expression of Tim-3, granzyme B, perforin, IFN-gamma and Fas-ligand mRNA by real-time RT-PCR, with probes and primers TaqMan. Protocol biopsies were studied as controls. Statistical analyses were performed to compare groups, and to investigate the potential association with gene transcripts measures and rejection. RESULTS: All molecules studied were up-regulated in the rejection group compared with controls (p<0.001). Acute rejection type III (Banff 97) profiles were associated with higher values, where granzyme B and perforin presented the highest (5672.51+/-9002.16 and 1866.59+/-2426.38, respectively) and Tim-3 had the lowest ones (166.62+/-174.94). Tim-3 had also a lower expression in those patients that did not respond to anti-rejection therapy. There was a positive correlation between Tim-3 and IFN-gamma mRNA expression levels (r(2)=0.73; p<0.001). CONCLUSIONS: Our results corroborate the concept that acute rejection is an active process, where inflammatory as well as regulatory factors have their roles. Severe episodes of acute rejection were associated with higher expression of cytotoxic molecules and lower expression of potential regulatory molecule.
PMID: 17331850 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 同种异体移植肾的Tim-3的表达.
作者： onciano VC, Renesto PG, Nogueira E, Rangel EB, Cenedeze MA, Franco MF, Camara NO, Pacheco-Silva A.（尊重原名，不予以翻译）
来源： 移植免疫 2007年4月，第17卷第3期，215-222页
摘要
研究背景：Tim-3 最近被认为是TH1（辅助性T细胞-1）特异性分子，参与免疫应答的调节和移植肾的耐受。这里我们在被排斥的移植肾上研究T细胞活化与细胞毒性的分子标记物和Tim-3的mRNA的的表达。
方法：20例因急性排斥反应问题、已经接受肾切除术、切除的移植肾被列入研究。我们利用探针、引物、RT-PCR技术定量测定标本中的Tim-3、粒酶B、穿孔素、γ-干扰素、和Fasl mRNA的量，诊断性穿刺活检作为对照组。组间比较进行统计学分析来探究被排斥移植肾和基因转录量之间的潜在联系。
结果：同对照组相比所有被排斥的移植肾的分子标记物均呈上调表达，在III型急性排斥反应（Banff标准，97年）表现尤为明显，粒酶B和穿孔素表达最高（分别为5672.51+/-9002.16 和1866.59+/-2426.38），而Tim-3的表达最低（166.62+/-174.94），同时Tim-3在对抗排斥治疗不敏感的患者（的移植肾）中也呈较低的表达。在mRNA水平Tim-3和γ-干扰素呈正相关(r(2)=0.73; p<0.001)。
结论：我们的结果证实急性排斥反应是一个活动进程，炎症性反应和调节因子均在其中起到作用。严重的急性排斥反应和较高的细胞毒性分子表达和较低的潜在的调节因子的表达相关。
编译后：（380字）
ONCIANO等发现Tim-3的调节可能有助于移植肾存活，这一观点发表在移植免疫2007年4月第17卷第3期上。
Tim-3 最近被认为是TH1（辅助性T细胞-1）特异性分子，参与免疫应答的调节和移植肾的耐受，ONCIANO等研究了20例因发生难以逆转的急性排斥反应而切除的移植肾和诊断性穿刺活检的肾脏标本，用探针、引物、RT-PCR技术定量测定了其中有关T细胞活化与细胞毒性的分子标记物如粒酶B、穿孔素、γ-干扰素、Fasl mRNA和Tim-3的mRNA的量，发现发生移植肾有关T活化和细胞毒性的分子活性明显上升，在III型急性排斥反应（Banff标准）表现尤为明显，其中粒酶B和穿孔素表达最高（分别为5672.51+/-9002.16 和1866.59+/-2426.38），而Tim-3的表达最低（166.62+/-174.94），且Tim-3在对抗排斥治疗无反应的移植肾中也呈较低的表达，但在mRNA水平Tim-3和γ-干扰素呈正相关(p<0.001)。作者认为，急性免疫排斥反应是炎症性因子和免疫调节因子共同作用的结果，严重的急性排斥反应与细胞毒性分子活性增高和潜在的调节因子活性减低有关。 请按文摘类新闻格式编译。 提出一点意见:Twenty human kidney grafts that had undergone nephrectomy due to an irreversible acute rejection episode were studied. 20例因发生难以逆转的急性排斥反应而切除的移植肾被列入研究。 有一点疑问：总体看来，Tim-3是有利于移植肾存活的，但文中提到“Tim-3 had also a lower expression in those patients that did not respond to anti-rejection therapy. ” 似乎与其矛盾，不知道怎么理解！ 难治性排斥反应，一般而言体液性免疫应答水平较高，免疫应答强度较高，因此，有利于耐受的因子表达水平低是可以理解的。 Tim-3 had also a lower expression in those patients that did not respond to anti-rejection therapy.
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作者:admin@医学,生命科学 2010-11-14 17:11
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