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【medical-news】CD33(+) CD14(−)表型是骨巨细胞瘤
Abstract
Giant Cell Tumour of Bone (GCT is a benign bone tumour with a demonstrated clinical behaviour of local recurrences and rare distant metastases. GCTB is composed of uniformly distributed osteoclastic giant cells, thought to originate from the fusion of monocyte-macrophage lineage cells, in a background consisting of mononuclear rounded cells and spindle-shaped cells. Several reports revealed the specific expression of markers, such as CD14 on the mononuclear rounded cell population, however lacking on osteoclastic giant cells. Blood monocytes which were CD14+, CD33+ or CD14+/CD33+ have also been shown to be programmed as pre-osteoclasts. The macrophage marker CD33 is expressed earlier than CD14 in macrophage maturation while CD14 is expressed longer than CD33. The aim of this study was to investigate CD14 / CD33 expression profiles in GCTB.
19 GCTB tumour samples of 19 patients were studied. Immunofluorescent analyses were performed with monoclonal antibodies against CD14, CD33, RANK and CD51. In order to unambiguously further prove the expression of these molecules quantitative RT-PCR was employed with subsequent sequencing of its products.
All samples showed similar immunoreactivity profiles. The mononuclear rounded cell population was positive for RANK, CD51, CD14 and CD33. The osteoclastic giant cell population expressed RANK and CD51, as well as CD33, but was consistently negative for CD14 expression. The CD14 en CD33 profiles were confirmed by quantitative RT-PCR. These RT-PCR products were sequence verified.
Osteoclasts in GCTB are the result of fusion of CD33-expressing pre-osteoclasts that further fuse with CD14+ mononuclear cells. Although these results reflect a static rather than a dynamic spectrum, we strongly believe that osteoclastogenesis seems not to be the exclusive result of fusion of intra-tumoural CD14+ mononuclear cells. Moreover, CD33-modulated osteoclastogenesis opens up the possibility for novel therapeutic directions.
Journal of Bone and Mineral Research, Published online September 3, 2008:10.1359/jbmr.080905
chengtao981003 wrote:
CD33(+) CD14(− Phenotype is Characteristic of Multinuclear Osteoclast-Like Cells in Giant Cell Tumor of Bone
Abstract
Giant Cell Tumour of Bone (GCT is a benign bone tumour with a demonstrated clinical behaviour of local recurrences and rare distant metastases. GCTB is composed of uniformly distributed osteoclastic giant cells, thought to originate from the fusion of monocyte-macrophage lineage cells, in a background consisting of mononuclear rounded cells and spindle-shaped cells. Several reports revealed the specific expression of markers, such as CD14 on the mononuclear rounded cell population, however lacking on osteoclastic giant cells. Blood monocytes which were CD14+, CD33+ or CD14+/CD33+ have also been shown to be programmed as pre-osteoclasts. The macrophage marker CD33 is expressed earlier than CD14 in macrophage maturation while CD14 is expressed longer than CD33. The aim of this study was to investigate CD14 / CD33 expression profiles in GCTB.
19 GCTB tumour samples of 19 patients were studied. Immunofluorescent analyses were performed with monoclonal antibodies against CD14, CD33, RANK and CD51. In order to unambiguously further prove the expression of these molecules quantitative RT-PCR was employed with subsequent sequencing of its products.
All samples showed similar immunoreactivity profiles. The mononuclear rounded cell population was positive for RANK, CD51, CD14 and CD33. The osteoclastic giant cell population expressed RANK and CD51, as well as CD33, but was consistently negative for CD14 expression. The CD14 en CD33 profiles were confirmed by quantitative RT-PCR. These RT-PCR products were sequence verified.
Osteoclasts in GCTB are the result of fusion of CD33-expressing pre-osteoclasts that further fuse with CD14+ mononuclear cells. Although these results reflect a static rather than a dynamic spectrum, we strongly believe that osteoclastogenesis seems not to be the exclusive result of fusion of intra-tumoural CD14+ mononuclear cells. Moreover, CD33-modulated osteoclastogenesis opens up the possibility for novel therapeutic directions.
Journal of Bone and Mineral Research, Published online September 3, 2008:10.1359/jbmr.080905
CD33(+) CD14(− Phenotype is Characteristic of Multinuclear Osteoclast-Like Cells in Giant Cell Tumor of Bone
CD33 （ + ）细胞CD14 （ -表型的特点是多核破骨细胞样细胞在骨巨细胞瘤
Abstract
摘要
Giant Cell Tumour of Bone (GCT is a benign bone tumour with a demonstrated clinical behaviour of local recurrences and rare distant metastases. GCTB is composed of uniformly distributed osteoclastic giant cells, thought to originate from the fusion of monocyte-macrophage lineage cells, in a background consisting of mononuclear rounded cells and spindle-shaped cells. Several reports revealed the specific expression of markers, such as CD14 on the mononuclear rounded cell population, however lacking on osteoclastic giant cells. Blood monocytes which were CD14+, CD33+ or CD14+/CD33+ have also been shown to be programmed as pre-osteoclasts. The macrophage marker CD33 is expressed earlier than CD14 in macrophage maturation while CD14 is expressed longer than CD33. The aim of this study was to investigate CD14 / CD33 expression profiles in GCTB.阅读本文的人还阅读：
作者:admin@医学,生命科学 2010-10-18 05:11
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