揭示艾滋发病新机理艾滋病毒研究再获重要进展

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揭示艾滋发病新机理艾滋病毒研究再获重要进展

于晓方在美国《科学》杂志发表研究艾滋病的论文，揭示了艾滋发病新机理，他领导的研究小组发现了一组使HIV逃避人体细胞天然防御系统，并能在人体内大量复制的蛋白复合体———泛素连接酶系统，从而为预防和治疗艾滋病开辟了新的道路。该研究在国际上首先发现一组使HIV避过人体细胞天然防御系统而在人体细胞内大量复制的蛋白，阐明了HIV突破宿主细胞防御系统的分子机制。于晓方教授解释说，艾滋病病毒含有一种病毒感染因子，该因子通过泛素连接酶共同作用，增加了艾滋病的感染机会。“我们通过一系列实验发现，只要阻断病毒感染因子和泛素连接酶的共同作用，艾滋病的感染率就降低了90%。这个新发现有可能使我们开发出防治HIV感染的新药和新疗法。

摘自今日http://www.ebiotrade.com/newsf/readnews.asp?recordno=L2003121294720

Fig Identification of cellular proteins that interact with HIV-1 Vif.
(A) Coimmunoprecipitation of cellular proteins with Vif-HA. Cell lysates from HXB2- or HXB2VifHA-infected H9 cells were immunoprecipitated with HA antibody, followed by SDS–polyacrylamide gel electrophoresis (PAGE) and silver staining. The identification of Cul5, elongin B, and elongin C was achieved by mass spectroscopic analysis; these proteins were not detected in the control HXB2 samples. kDa, kilodaltons. ( Immunoblot of precipitated samples from HXB2 and HXB2VifHA-infected H9 cells. Precipitated samples as described above were separated by SDS-PAGE, transferred to nitrocellulose membranes, and reacted with antibodies against Cul5, HIV-1 Vif, elongin B, Rbx1, or elongin C. (C) Coimmunoprecipitation of HIV-1 Vif with Cul5. The 293T cells were cotransfected with HXB2 plus Cul5-HA or Cul5-Myc expression vectors. Cell lysates were prepared 48 hours after transfection and immunoprecipitated with HA antibody. Proteins in cell lysates and immunoprecipitated samples were detected by immunoblotting with the use of antibodies against Cul5, Vif, elongin B, or elongin C. IP, immunoprecipitation. (D) Reduced interaction of VifSLQ with Cul5-SCF. Cell lysates from HXB2VifHA- or HXB2VifSLQ-HA–infected H9 cells were immunoprecipitated with HA antibody. Precipitated samples were analyzed by immunoblotting with antibodies against Cul5, HIV-1 Vif, elongin B, or elongin C.

详见Science全文
http://www.sciencemag.org/cgi/content/full/302/5647/1056 [标签:content1][标签:content2]

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作者:admin@医学,生命科学 2011-06-28 05:45
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