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【Gut】人胰腺癌疗法研究取得新进展

郑州大学中英分子肿瘤学研究中心继去年承担国家自然科学基金重点项目,研究成果发表在国际一流期刊Journal of Clinical Investigation后,姜国忠和曹风雨为共同第一作者,中英分子肿瘤学中心科技主管王尧河和中心主任尼克莱蒙为通讯作者的研究论文“PRSS3 Promotes Tumour Growth and Metastasis of Human Pancreatic Cancer”近日在世界胃肠肝病学的顶级杂志《内脏》(Gut)上发表。

该研究发现,作为胰蛋白酶原家族一员的PRSS3通过PAR-1介导ERK磷酸化导致VEGF在肿瘤细胞中高表达,从而促进肿瘤细胞的生长和转移,该信号传导通路的发现为我们提供了PRSS3促进肿瘤进展和转移的理论依据,同时为人胰腺癌的治疗开辟了一条新思路。(来源:科学时报 谭永江 肖霞) Abstract
Background and aims Metastasis accounts for the poor outcome of patients with pancreatic cancer. We recently discovered PRSS3 to be over-expressed in metastatic human pancreatic cancer cells. This study aimed to elucidate the role of PRSS3 in the growth and metastasis of human pancreatic cancer.

Methods PRSS3 expression in human pancreatic cancer cell lines was detected by qPCR and immunoblotting. The effect of PRSS3 on cancer cell proliferation, migration and invasion in vitro, tumour growth and metastasis in vivo were investigated by manipulation of PRSS3 expression in human pancreatic cancer cell lines. VEGF expression was detected by ELISA, and the pathway through which PRSS3 regulates VEGF expression was investigated. The therapeutic effect of targeting this pathway on metastasis was assessed in vivo. Immunohistochemistry was employed to detect PRSS3 expression in human pancreatic cancer tissues.

Results PRSS3 was over-expressed in the metastatic PaTu8988s cell line, but not in the non-metastatic PaTu8988t cell line. Over-expression of PRSS3 promoted pancreatic cancer cell proliferation as well as invasion in vitro, and tumour progression and metastasis in vivo. Stepwise investigations demonstrated that PRSS3 upregulates VEGF expression via the PAR1-mediated ERK pathway. ERK inhibitor significantly delayed the progression of metastases of pancreatic cancer and prolonged the survival of animals bearing metastatic pancreatic cancer (p<0.05). 40.54% of human pancreatic cancers (n=74) were positive for PRSS3 protein. A significant correlation was observed between PRSS3 expression and metastasis (p<0.01). Multivariate Cox regression analysis indicated that patients with PRSS3 expression in their tumours had a shorter survival time compared to those without PRSS3 expression (p<0.05).

Conclusion PRSS3 plays an important role in the progression, metastasis and prognosis of human pancreatic cancer. Targeting the PRSS3 signalling pathway may be an effective and feasible approach for treatment of this lethal cancer.
http://gut.bmj.com/content/59/11/1535.short?rss=1 欢迎大家积极提供生物及生命工程信息 - 业界热点资讯版 - 爱爱...发表时间:2010-12-19回帖数:0生物及医学新闻我们必须客观科学面对,不能来半点需要,这是做医生起码原则,现在我欢迎大家提供提供生物及生命工程信息包括:会议,研究进展,必须具有真实性,实际性,创造性,创新性...www.iiyi.com/bbs/viewthread... - 2011-01-08-快照-生命工程信息 - 业界热点资讯版 - 爱爱..." 2009 年 全国肿瘤登记工作经验交流会文件类型:PDF/Adobe Acrobat- 文字版上海市肿瘤登记质量评价研究. 儿童恶性肿瘤流行病学研究进展. 7 肿瘤登记 2009 ... 掌握全市肿瘤发病和死亡动态,为政府制定肿瘤 防治策略提供科学依据. (二)各乡镇卫生...www.cicams.ac.cn/...531941.pdf-2011-01-14-实验研究:泰式咖哩香料可对抗癌细胞根据加拿大科学家最新的研究结果,多吃蔬菜和水果有助於预防胰腺癌.在这项研究中,研... 我国近两年在医药领域的研究已经取得一定的进展,主要集中在纳米药物载体治疗与诊断技...www.taishan-international.org/-2005-04-01-快照-研究:泰式咖哩香料可对抗癌细胞"[标签:content2]

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作者:admin@医学,生命科学    2011-01-26 23:40
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