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【medical-news】黄素蛋白自体荧光检测法有助于糖
"Hyperglycemia induces mitochondrial stress and apoptotic cell death in diabetic tissues soon after disease onset and before involvement can be detected by any current clinical diagnostic method," write Matthew G. Field, from the University of Michigan in Ann Arbor, and colleagues. "Many subjects with diabetes may remain undiagnosed until they develop diabetic microvascular and macrovascular complications. A noninvasive method of measuring FA to detect early ocular dysfunction due to disease has been previously described."
The goal of this study was to determine, using a rapid, noninvasive clinical imaging method, whether individuals with diabetes have enhanced retinal FA compared with age-matched individuals without diabetes.
Retinal imaging using 1-millisecond flashes of 467-nm light was compared in 21 subjects with diabetes mellitus and in 21 healthy age-matched control volunteers. For each flash at 535 nm, FA was recorded using an electron-multiplying charged coupled device camera with a 512 x 512-pixel chip. Analysis of histograms of pixel intensities plotted for each eye allowed determination of the average intensity and the average curve width of retinal FA.
Across all 3 consecutive decades of life studied, the mean average intensity and average curve width levels in participants with diabetes were significantly greater than those in the control group, when stratified by age (P ≤ .004 and P ≤ .006, respectively). Comparing the mean average intensity and average curve width levels in all participants with diabetes vs all controls, after adjusting for age, was consistent with the results found in each age category (P = .001 and P < .001, respectively).
Compared with participants with diabetes but without retinopathy in either eye, those with diabetes and with retinopathy in at least 1 eye had significantly greater average intensity and average curve width (P = .002 and P = .005, respectively).
"Flavoprotein autofluorescence measurements may be clinically useful to rapidly and noninvasively identify diabetic metabolic tissue stress and disease severity," the study authors write. "Development of flavoprotein autofluorescence technology is likely to result in a tool that will improve diabetes screening and disease management."
Limitations of this study include small sample size and the effects of potentially confounding ocular fluorophores on FA measurements.
"Unlike glucose monitoring, elevations in FA reflect ongoing diabetic tissue damage and may provide patient and caregiver motivation for intensifying disease management," the study authors conclude.
The National Eye Institute, National Institutes of Health and a Research to Prevent Blindness Senior Scientific Investigator Award supported this study. Two authors have disclosed having founded OcuSciences, Inc, to commercialize the technology.
Arch Ophthalmol. 2008;126:934-938. [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-08-05 06:26
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