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【bio-news】强大的人类基因组鉴定新方法
For cancer biology and other medical applications, optical mapping reveals more than traditional DNA sequencing
A mathematical discovery has extended the reach of a novel genome mapping method to humans, potentially giving cancer biology a faster and more cost-effective tool than traditional DNA sequencing.
A student-led group from the laboratory of Michael Waterman, USC University Professor in molecular and computational biology, has developed an algorithm to handle the massive amounts of data created by a restriction mapping technology known as "optical mapping." Restriction maps provide coordinates on chromosomes analogous to mile markers on freeways.
Lead author Anton Valouev, a recent graduate of Waterman's lab and now a postdoctoral fellow at Stanford University, said the algorithm makes it possible to optically map the human genome.
"It carries tremendous benefits for medical applications, specifically for finding genomic abnormalities," he said.
The algorithm appears in this week's PNAS Early Edition.
Optical mapping was developed at New York University in the late 1990s by David Schwartz, now a professor of chemistry and genetics at the University of Wisconsin-Madison. Schwartz and a collaborator at Wisconsin, Shiguo Zhou, co-authored the PNAS paper.
The power of optical mapping lies in its ability to reveal the size and large-scale structure of a genome. The method uses fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes.
By imaging large numbers of an organism's DNA molecules, optical mapping can produce a map of its genome at a relatively low cost.
An optical map lacks the minute detail of a genetic sequence, but it makes up for that shortcoming in other ways, said Philip Green, a professor of genome sciences at the University of Washington who edited the PNAS paper.
Geneticists often say that humans have 99.9 percent of their DNA in common. But, Green said, "individuals occasionally have big differences in their chromosome structure. You sometimes find regions where there are larger changes."
Such changes could include wholesale deletions of chunks of the genome or additions of extra copies. Cancer genomes, in particular, mutate rapidly and contain frequent abnormalities.
"That's something that's very hard to detect" by conventional sequencing, Green said, adding that sequencing can simply miss part of a genome.
Optical mapping, by contrast, can estimate the absolute length of a genome and quickly detect differences in length and structure between two genomes. Comparing optical maps of healthy and diseased genomes can guide researchers to crucial mutations.
Though he called optical mapping "potentially very powerful," Green added that it requires such a high level of expertise that only a couple of laboratories in the world use the method.
The Waterman group's algorithm may encourage others to take a second look.
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Funding for the group's research came from the National Institutes of Health, the National Science Foundation and the Preuss Foundation.
OPTICAL.MAPPING.CM --USC-- OCT. 10, 2006 认领 这篇文章 Powerful genome ID method extended to humans
强大的人类基因组鉴定新方法
For cancer biology and other medical applications, optical mapping reveals more than traditional DNA sequencing
在癌症生物医学以及其他医学应用领域上，光学标测较传统 DNA序列分析显示出了优势。
A mathematical discovery has extended the reach of a novel genome mapping method to humans, potentially giving cancer biology a faster and more cost-effective tool than traditional DNA sequencing.
一项数学发现已经应用到新的人类基因组定位方法的研究上来，可能给癌症生物学带来比传统 DNA序列分析更快、更廉价而有效的研究工具。
A student-led group from the laboratory of Michael Waterman, USC University Professor in molecular and computational biology, has developed an algorithm to handle the massive amounts of data created by a restriction mapping technology known as "optical mapping." Restriction maps provide coordinates on chromosomes analogous to mile markers on freeways.
Michael Waterman实验室的学生研究组、南加州大学分子生物学和计算机生物学教授已经发现了应用限制性内切酶谱技术处理大量数据的法则，该法被命名为“光学标测”。
Lead author Anton Valouev, a recent graduate of Waterman's lab and now a postdoctoral fellow at Stanford University, said the algorithm makes it possible to optically map the human genome.
主要作者 Anton Valouev（刚毕业于 Waterman实验室，现在在斯坦福大学从事博士后工作）说，该法则使光学标测人类基因组成为可能。
"It carries tremendous benefits for medical applications, specifically for finding genomic abnormalities," he said.
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作者:admin@医学,生命科学 2011-07-30 17:30
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