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【文摘发布】血管内机制参与白蛋白对急性脑缺
A Two-Photon Laser-Scanning Microscopy Study
Anitha Nimmagadda, MD; Hee-Pyoung Park, MD, PhD; Ricardo Prado, MD Myron D. Ginsberg, MD
From the Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami Miller School of Medicine, Miami, Fla, USA.
Background and Purpose— High-dose human albumin is robustly neuroprotective in preclinical ischemia models and is currently in phase III clinical trial for acute ischemic stroke. To explore the hypothesis that albumin’s protective effect is mediated in part by salutary intravascular mechanisms, we assessed microvascular hemodynamics in a model of laser-induced cortical arteriolar thrombosis.
Methods— The cortical microcirculation of anesthetized, physiologically monitored Sprague-Dawley rats was studied in vivo via a frontoparietal cranial window (intact dura) by two-photon laser-scanning microscopy after plasma-labeling with fluorescein-dextran. Focal thrombosis was produced in 30- to 50-µm cortical arterioles by laser irradiation. Arteriolar flow velocity was measured repeatedly by line-scanning. At 30 minutes post-thrombosis, animals were treated with either human albumin, 2 g/kg, or with saline control.
Results— Baseline arteriolar flow velocity averaged 3.5±1.8 mm/s and was reduced to 10% to 13% of control values by laser-induced thrombosis, which also led to focal vasodilatation (mean, 49% above baseline diameter). Saline treatment at 30 minutes post-thrombosis failed to influence arteriolar flow velocity, which remained depressed at 10% to 22% of control throughout the subsequent 60- to 90-minute observation period. By contrast, albumin treatment induced a prompt rise in median flow velocity to 38% of control by 10 minutes post-treatment, and to 61% to 67% of control by 50 to 60 minutes.
Conclusions— High-dose albumin therapy induces a prompt, sustained improvement in microvascular hemodynamics distal to a cortical arteriolar thrombosis; these data support an important intravascular component to albumin’s protective effect in acute cerebral ischemia.
Stroke. 2008;39:198-204 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Albumin Therapy Improves Local Vascular Dynamics in a Rat Model of Primary Microvascular Thrombosis
白蛋白疗法改善大鼠原发微血管栓塞模型中的局部血流动力学
A Two-Photon Laser-Scanning Microscopy Study
一项"双光子激光扫描"显微镜研究
Anitha Nimmagadda, MD; Hee-Pyoung Park, MD, PhD; Ricardo Prado, MD Myron D. Ginsberg, MD
From the Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami Miller School of Medicine, Miami, Fla, USA.
美国.佛罗里达州.迈阿密城,迈阿密.米勒大学医学院,神经病学分部,脑血管病研究中心消息.
Background and Purpose— High-dose human albumin is robustly neuroprotective in preclinical ischemia models and is currently in phase III clinical trial for acute ischemic stroke. To explore the hypothesis that albumin’s protective effect is mediated in part by salutary intravascular mechanisms, we assessed microvascular hemodynamics in a model of laser-induced cortical arteriolar thrombosis.
背景及目的:大量人血白蛋白对临床前缺血模型有着稳固的神经保护作用,目前已处急性缺血性中风研究第3期的临床试验阶段.为了探究白蛋白保护效应部分由良性血管内机制所介导的假说,我们评估了激光激励皮质血管栓塞模型中的微血管血流动力学的改变.
Methods— The cortical microcirculation of anesthetized, physiologically monitored Sprague-Dawley rats was studied in vivo via a frontoparietal cranial window (intact dura) by two-photon laser-scanning microscopy after plasma-labeling with fluorescein-dextran. Focal thrombosis was produced in 30- to 50-µm cortical arterioles by laser irradiation. Arteriolar flow velocity was measured repeatedly by line-scanning. At 30 minutes post-thrombosis, animals were treated with either human albumin, 2 g/kg, or with saline control.
方法:麻醉并生理监测活体Sprague-Dawley大鼠,荧光葡聚糖标记其脑皮质微循环,双光子激光扫描显微镜下通过额顶颅窗(硬膜完整)观察研究.通过激光辐射产生小动脉内30-50微米的血栓栓子.通过线样扫描反复检测小动脉血液流速.栓塞后30钟,给动物使用2 g/kg的人血白蛋白或盐水对照.
Results— Baseline arteriolar flow velocity averaged 3.5±1.8 mm/s and was reduced to 10% to 13% of control values by laser-induced thrombosis, which also led to focal vasodilatation (mean, 49% above baseline diameter). Saline treatment at 30 minutes post-thrombosis failed to influence arteriolar flow velocity, which remained depressed at 10% to 22% of control throughout the subsequent 60- to 90-minute observation period. By contrast, albumin treatment induced a prompt rise in median flow velocity to 38% of control by 10 minutes post-treatment, and to 61% to 67% of control by 50 to 60 minutes.
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作者:admin@医学,生命科学 2011-07-21 18:16
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