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【Cell Biochemistry & Biophysics——编译】直流电可以
Exposure of Human Leukemic Cells to Direct Electric Current: Generation of Toxic Compounds Inducing Cell Death by Different Mechanisms.[Article]
来源
Cell Biochemistry & Biophysics. 42(1):61-74, 2005.
Accession Number
00063476-200542010-00006.
Author
Veiga, Venicio F. 1; Nimrichter, Leonardo 1; Teixeira, Cesar A. 3; Morales, Marcelo M. 2; Alviano, Celuta S. 1; Rodrigues, Marcio L. 1; Holandino, Carla 3,*
Institution
(1)Instituto de Microbiologia Professor Paulo de Goes, Universidade Federal do Rio de Janeiro, CCS, Bloco K, Segundo Andar, Sala 50, Ilha do Fundao, 21941-590, Rio de Janeiro, Brazil
(2)Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Bloco K, Segundo Andar, Sala 50, Ilha do Fundao, 21941-590, Rio de Janeiro, Brazil
(3)Departamento de Medicamentos-Faculdade de Farmacia, Centro de Ciencias da Saude, Universidade Federal do Rio de Janeiro, CCS, Bloco K, Segundo Andar, Sala 50, Ilha do Fundao, 21941-590, Rio de Janeiro, Brazil
摘要原文
Treatment with direct electric current (DC) influences the growth of several cancer cells. In this work, we evaluated the effects of DC treatment on the human leukemic cell line HL60. Human cells were separately treated in the presence of the cathode or the anode or without contact with the electrodes. In all systems, DCtreated cells presented an impaired ability to proliferate. Growth inhibition was dependent on the generation of soluble products of electrolysis. Cathodic treatment of HL60 cells predominantly induced lysis, whereas treatment without contact with electrodes did not induce alterations in cell viability. In contrast, cell stimulation by the anode resulted in irreversible membrane damage, as demonstrated by trypan blue and 7-aminoactinomycin staining. Analysis of these cells by transmission electron microscopy indicated that necrosis is a major mechanism inducing cell death. In addition, apoptotic-like cells were observed under light microscopy after anodic treatment. Accordingly, DNA from anodic-treated cells presented a typical pattern of apoptosis. Apoptotic cell death was only generated after the treatment of HL60 cells in conditions in which the generation of chloride-derived compounds was favored. These results indicate that the nature of the products from cathodic or anodic reactions differently influences the mechanisms of cell death induced by DC-derived toxic compounds.
编译
给与直流电（DC）可以影响几种癌细胞生长。这份论文评估了人白血病细胞株HL60给予DC的效果。人细胞分别置于正极、负极或不予电极接触。所有系统中，给予DC后，细胞表现出增殖能力的损害。负极下的HL60细胞主要诱导细胞溶解，而不予电极接触不会诱导细胞生存能力的变化。相反，正极刺激细胞导致不可逆的膜损害，这通过锥虫蓝和7-氨基放线菌素染色说明。通过透射电子显微镜分析这些细胞，显示坏死是诱导细胞死亡的主要机制。另外，给予正极刺激后，显微镜下观察到了凋亡相似的细胞。因此，给予正极的细胞DNA呈现典型的凋亡特征。凋亡细胞死亡偏向于发生在氯化物衍生的化合物产生的情况下。这些结果说明，通过DC诱导衍生的毒性化合物在正或负极反应下对细胞死亡有不同影响机制。 直流电可以诱发癌细胞死亡
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作者:admin@医学,生命科学 2011-04-18 17:14
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