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疟原虫文摘学习
设计了一下基本格式：
1.编号
2.文题
3.作者
4.杂志
5.卷期页码
6.年份
7.文摘
8.文摘译文
9.全文链接
10.学习体会
11.疑问
目录： 1.编号：0001
2.文题：Mapping the anatomy of a Plasmodium falciparum MSP-1
epitope using pseudopeptide-induced mono- and polyclonal
antibodies and CD and NMR conformation analysis
3.作者：Lozano JM, Espejo F, Ocampo M,et al.
4.杂志：J Struct Biol.
5.卷期页码：148(1):110-22
6.年份：2004 Oct
7.文摘：Antigen structure modulation represents an approach towards designing subunit malaria vaccines. A specific epitope's alpha carbon stereochemistry, as well as its backbone topochemistry, was assessed for obtaining novel malarial immunogens. A variety of MSP-1(38-61) Plasmodium falciparum epitope pseudopeptides derived were synthesised, based on solid-phase pseudopeptide chemistry strategies; these included all-L, all-D, partially-D substituted, all-Psi-[NH-CO]-Retro, all-Psi-[NH-CO]-Retro-inverso, and Psi-[CH2NH] reduced amide surrogates. We demonstrate that specific recombinant MSP-1(34-469) fragment binding to red blood cells (RBCs) is specifically inhibited by non-modified MSP-1(42-61), as well as by its V52-L53, M51-V52 reduced amide surrogates and partial-D substitutions in K48 and E49. In vivo tests revealed that reduced amide pseudopeptide-immunised Aotus monkeys induced neutralising antibodies specifically recognising the MSP-1 N-terminus region. These findings support the role of molecular conformation in malaria vaccine development.
8.文摘译文：抗原的结构调整是设计亚单位疟疾疫苗的一种方法。我们通过评价特异性表位
碳原子的文体化学及其碳骨架的拓扑化学以期获得新的疟疾抗原。基于固相假想肽化学策略，我们合成了多种来源于恶性疟原虫MSP-1（38-61）表位的假想肽，这些假想肽包括全L型、全D型、部分D型置换，全型[NH-CO]回复、全Psi型[NH-CO]反向回复和Psi型[NH-CO]还原的酰胺替代物。实验表明，与V52-L53、M51-V52位还原性酰胺替代物和K48-E59位部分D型置换一样，未经修饰的MSP-1（42-61）特异性抑制MSP-1（34-469）片断与红细胞的特异性结合。体内试验提示，还原性酰胺假想肽免疫的夜猴中可诱导出中和性抗体，这些抗体特异性识别MSP-1的N末端区域。这些结果证实了在疟疾疫苗的研制中分子构象发挥的作用。
9.全文链接：
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15363791&query_hl=3
10.学习体会
11.疑问 ：pseudopeptide中文用什么来解释合适呢， 1.编号：0002
2.文题：Characterization of Conserved T- and B-Cell Epitopes in Plasmodium falciparum Major Merozoite Surface Protein 1
3.作者：Parra M, Hui G, Johnson AH,et al.
4.杂志：INFECTION AND IMMUNITY
5.卷期页码：68（5）:2685-2691
6.年份：2000 May
7.文摘：Vaccines for P. falciparum will need to contain both T- and B-cell epitopes. Conserved epitopes are the most desirable, but they are often poorly immunogenic. The major merozoite surface protein 1 (MSP-1) is currently a leading vaccine candidate antigen. In this study, six peptides from conserved or partly conserved regions of MSP-1 were evaluated for immunogenicity in B10 congenic mice. Following immunization with the peptides, murine T cells were tested for the ability to proliferate in vitro and antibody responses to MSP-1 were evaluated in vivo. The results showed that one highly conserved sequence (MSP-1#1, VTHESYQELVKKLEALEDAV; located at amino acid positions 20 to 39) and one partly conserved sequence (MSP-1#23, GLFHKEKMIL NEEEITTKGA; located at positions 44 to 63) contained both T- and B-cell epitopes. Immunization of mice with these peptides resulted in T-cell proliferation and enhanced production of antibody to MSP-1 upon exposure to merozoites. MSP-1#1 stimulated T-cell responses in three of the six strains of mice evaluated, whereas MSP-1#23 was immunogenic in only one strain. Immunization with the other four peptides resulted in T-cell responses to the peptides, but none of the resulting peptide-specific T cells recognized native MSP-1. These results demonstrate that two sequences located in the N terminus of MSP-1 can induce T- and B-cell responses following immunization in a murine model. Clearly, these sequences merit further consideration for inclusion in a vaccine for malaria.
8.文摘译文： 针对恶性疟原虫的疫苗需要同时包含T细胞表位和B细胞表位。保守性表位是最理想化的，但是其免疫原性通常很弱。主要裂殖子表面蛋白1（MSP-1）是当前领先的疫苗候选抗原。本研究测量了B10同类系小鼠中六个来源于MSP-1的保守或部分保守性肽的免疫原性。用肽免疫后，我们测量鼠T细胞以了解其体外增殖能力，并测量MSP-1的体内抗体反应。结果提示：一个高度保守序列(MSP-1#1, VTHESYQELVKKLEALEDAV; 位于第20至39位氨基酸)和一个部分保守序列(MSP-1#23, GLFHKEKMIL NEEEITTKGA;位于第44至63位氨基酸)同时包含T细胞表位和B细胞表位。用这些表位肽免疫小鼠后，T细胞发生增殖并且暴露于裂殖子时针对MSP-1的抗体增加。MSP-1#1在小鼠中测量了六株，在其中的三株中检测到了T细胞反应，而MSP-1#23仅在一株中表现出了免疫原性。用其它四条肽链免疫后出现了针对该肽的T细胞反应，但是产生的肽特异性T细胞都不识别天然MSP-1。结果表明在MSP-1N末端的两个序列可以在免疫小鼠模型后诱发T细胞和B细胞反应。显然，这些序列在进一步设计疟疾疫苗中具有参考价值。
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作者:admin@医学,生命科学 2011-03-10 05:12
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