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【bio-news】新方法加速遗传密码破译
US and Australian scientists have pioneered a new hybrid method for genomic sequencing that is faster and cheaper than state of the art technologies.
The breakthrough will be welcomed in medical and biotechnology circles where there is rising demand for genome-sequencing technologies. The new hybrid method combines the best of new and old code cracking methods for "fingerprinting" the genetic basis of life.
Scientists from the US-based J. Craig Venter Institute and the University of New South Wales in Sydney, Australia published the findings in the Proceedings of the National Academy.
"Cracking the entire, genetic code of an organism is expensive and until recently has relied in its fundamentals on a 30 year old technology that involves a physical separation of gene fragments," says Dr Torsten Thomas, a study co-author and senior research fellow at the University of New South Wales.
"A newer method which has emerged in the past year uses real-time, light-based observations of gene synthesis to reveal genomic information. It produces genomic information up 100 times faster than the old technology."
Using the genomes of six ocean bacteria, the scientists evaluated the utility and cost effectiveness of the old and new methods to show that a hybrid method was better than either method on its own. They found that combining the advantages of the two sequencing methods in a hybrid approach produced better quality genomic information.
The team found that the traditional method known as 'Sanger' sequencing worked best at sequencing large segments of the genomes, while the newer method known as '454 pyrosequencing' was more adept at sequencing smaller, more difficult sections, such as unclonable regions and gaps induced by secondary structures. The hybrid sequencing approach enabled the scientists to more easily close sequencing gaps between genome fragments compared with previous techniques.
The researchers suggest that the hybrid technique will become the preferred method for sequencing small microbial genomes, as the Sanger method is more capable of sequencing larger segments of DNA. "The new hybrid approach has generated exceptional results for several, marine microbes and we hope that our findings will kick-start other genome projects that were previously constraint by economic considerations", Dr Thomas says. 先认领了 水平有限，欢迎多多批评指正。
New tool cracks genomic code quicker than ever
新方法加速遗传密码破译
US and Australian scientists have pioneered a new hybrid method for genomic sequencing that is faster and cheaper than state of the art technologies.
美国和澳大利亚的科学家开辟了一种比最新技术更快更廉价的基因组测序的新的混合方法。
The breakthrough will be welcomed in medical and biotechnology circles where there is rising demand for genome-sequencing technologies. The new hybrid method combines the best of new and old code cracking methods for "fingerprinting" the genetic basis of life.
该项突破将在对基因组测序技术要求越来越高的医药和生物技术领域大受欢迎。此新方法吸取了新老密码破译方法的长处以解读生命的遗传基础。
Scientists from the US-based J. Craig Venter Institute and the University of New South Wales in Sydney, Australia published the findings in the Proceedings of the National Academy.
来自美国J. Craig Venter学会以及澳大利亚悉尼New South Wales大学的科学家们在国家学术会议上公布了这个发现。
"Cracking the entire, genetic code of an organism is expensive and until recently has relied in its fundamentals on a 30 year old technology that involves a physical separation of gene fragments," says Dr Torsten Thomas, a study co-author and senior research fellow at the University of New South Wales.
"A newer method which has emerged in the past year uses real-time, light-based observations of gene synthesis to reveal genomic information. It produces genomic information up 100 times faster than the old technology."
New South Wales大学的资深研究合作伙伴Torsten Thomas博士讲到：“破译一个生物的全部遗传密码非常昂贵而且至今还要依赖30年前那种需要对基因片段进行物理分离才能完成的老技术。去年出现了一种实时的基于光学的观察法，可以通过直接观察基因合成来展示基因组信息。这项技术要比以往的快100倍。”
Using the genomes of six ocean bacteria, the scientists evaluated the utility and cost effectiveness of the old and new methods to show that a hybrid method was better than either method on its own. They found that combining the advantages of the two sequencing methods in a hybrid approach produced better quality genomic information.
利用六大洋细菌的基因组信息，科学家从新老方法的实用性和花销价值评估得出：这种混合方法优于其原来的任何一种。并发现在混合方法中集合两种测序方法的优点得到了更高质量的基因组信息。
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作者:admin@医学,生命科学 2011-02-19 17:14
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