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【bio-news】通过蛋白质转运改善肝干细胞的移植
Source: Society for Experimental Biology and Medicine
This release is available in Chinese.
Researchers at INSERM (France) have engineered a chimeric protein that increases cell survival, migration and proliferation to improve stem cell engraftment. The results, which appear in the September 2009 issue of Experimental Biology and Medicine, show that TAT-Tpr-Met, a cell permeable form of the hepatocyte growth factor receptor can increase the number of hepatic stem cells integrated into the liver of the mouse. TAT-Tpr-Met is the result of the fusion of Tpr-Met, an autoactived tyrosine kinase, to the protein transduction domain from HIV-TAT that gives the potential of the whole protein to enter into cells. TAT-Tpr-Met enters into cells in just one hour, where it then remains stable and recapitulates several effects seen with the hepatocyte growth factor (HGF). As the activating signal induced by TAT-Tpr-Met comes from inside the cells, it is independent of the extracellular environment, and continues even if cells are placed in vivo. This property was used to transplant cells with an advantage that allows cells pretreated with TAT-Tpr-Met to engraft twice more than untreated cells. The research team, Guillaume Kellermann, a graduate student in Biotechnology (University Paris VII), along with Lyes Boudechice, a veterinarian surgeon, Dr. Anne Weber and Dr. Michelle Hadchouel performed the studies in six week old mice. Dr. Kellermann noted that "previous work had already shown that cells engraft better with HGF, but contrary to other strategies, our method is virus and DNA free, so it may be safe for humans. However, before, considering clinical applications further studies need to be performed to check for the long term effects".
In summary, stem cells have an enormous potential in cell therapy, however their ability to engraft in solid tissues remains low. Here, a chimeric protein was engineered that switches them to a state more favorable to engraftment by promoting their survival, migration and proliferation. After a few days the protein is completely degraded inside the cells, therefore our method is safer than other strategies using modified virus and may be compatible with clinical applications.
Dr. Steven R. Goodman, Editor-in-Chief of Experimental Biology and Medicine, said "The authors have reported a very clever approach, utilizing the fusion protein TAT-Tpr-Met, to increase hepatic stem cell engraftment into the liver. The fact that the approach does not require virus or DNA makes it a reasonable strategy for future clinical applications." 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 它有机译，我就编辑一下好了，请指正
Improvement of liver stem cell engraftment by protein delivery
通过蛋白质转运改善肝干细胞移植效果
Source: Society for Experimental Biology and Medicine
This release is available in Chinese.
Researchers at INSERM (France) have engineered a chimeric protein that increases cell survival, migration and proliferation to improve stem cell engraftment.
法国INSERM研究人员设计出一种嵌合蛋白，可通过促进细胞的存活、迁移和增殖来提高干细胞移植效果。
The results, which appear in the September 2009 issue of Experimental Biology and Medicine, show that TAT-Tpr-Met, a cell permeable form of the hepatocyte growth factor receptor can increase the number of hepatic stem cells integrated into the liver of the mouse.
该项研究成果刊登在Experimental Biology and Medicine2009年9月份期刊上，结果显示TAT-Tpr-Met是一种可以穿透细胞膜且具有肝细胞生长因子受体功能的嵌合蛋白质，用于肝脏干细胞移植可增加进入小鼠肝脏的干细胞数目。
TAT-Tpr-Met is the result of the fusion of Tpr-Met, an autoactived tyrosine kinase, to the protein transduction domain from HIV-TAT that gives the potential of the whole protein to enter into cells.
TAT-Tpr-Met是一种融合蛋白质，结合具酪胺酸激酶活性的Tpr-Met和HIV-TAT的蛋白质转导域，因而可将整个蛋白质传送入细胞内。
TAT-Tpr-Met enters into cells in just one hour, where it then remains stable and recapitulates several effects seen with the hepatocyte growth factor (HGF).
TAT-Tpr-Met1小时内就可进入细胞，继而在细胞内稳定地停留并发挥肝细胞生长因子(HGF)的一些功能。
As the activating signal induced by TAT-Tpr-Met comes from inside the cells, it is independent of the extracellular environment, and continues even if cells are placed in vivo. This property was used to transplant cells with an advantage that allows cells pretreated with TAT-Tpr-Met to engraft twice more than untreated cells.
TAT-Tpr-Met的活化信号来自细胞内，因此它不受细胞外界环境的影响，即使在生物体内也能继续发挥作用，利用这个特性可以让TAT-Tpr-Met预处理过的细胞比不处理的细胞移入数量增加两倍。
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作者:admin@医学,生命科学 2010-11-12 05:11
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