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【文摘发布】让脂肪肝可用于肝移植的保存方法
Author:Maud Bessems , Benedict M. Doorschodt , Joe L.P. Kolkert , Reeta L. Vetelainen , Arlene K. van Vliet 1, Heleen Vreeling , Jan van Marle, Thomas M. van Gulik
Source:Liver Transpl. 2007 Apr;13(4):497-504
IF:4.447(2005)
Liver grafts are frequently discarded due to steatosis. Steatotic livers can be classified as suboptimal and deteriorate rapidly during hypothermic static preservation, often resulting in graft nonfunction. Hypothermic machine perfusion (MP) has been introduced for preservation of donor livers instead of cold storage (CS), resulting in superior preservation outcomes. The aim of this study was to compare CS and MP for preservation of the steatotic donor rat liver. Liver steatosis was induced in male Wistar rats by a choline-methionine-deficient diet. After 24 hours hypothermic CS using the University of Wisconsin solution (UW) or MP using UW-Gluconate (UW-G), liver damage (liver enzymes, perfusate flow, and hyaluronic acid clearance) and liver function (bile production, ammonia clearance, urea production, oxygen consumption, adenosine triphosphate [ATP] levels) were assessed in an isolated perfused rat liver model. Furthermore, liver biopsies were visualized by hematoxylin and eosin staining. Animals developed 30 to 60% steatosis. Livers preserved by CS sustained significantly more damage as compared to MP. Bile production, ammonia clearance, urea production, oxygen consumption, and ATP levels were significantly higher after MP as compared to CS. These results were confirmed by histology. In conclusion, MP improves preservation results of the steatotic rat liver, as compared to CS. Liver Transpl 本人认领此文。如在48小时内未能提交译文，其他战友可自由认领。 Titlereservation of steatotic livers: A comparison between cold storage and machine perfusion preservation
让脂肪肝可用于肝移植的保存方法---冷保存及低温机器灌注保存的比较
Author:Maud Bessems , Benedict M. Doorschodt , Joe L.P. Kolkert , Reeta L. Vetelainen , Arlene K. van Vliet 1, Heleen
Vreeling , Jan van Marle, Thomas M. van Gulik
作者：Maud Bessems , Benedict M. Doorschodt , Joe L.P. Kolkert , Reeta L. Vetelainen , Arlene K. van Vliet 1, Heleen Vreeling
, Jan van Marle, Thomas M. van Gulik
Source:Liver Transpl. 2007 Apr;13(4):497-504
来源：肝移植。2007 Apr;13(4):497-504
Liver grafts are frequently discarded due to steatosis. Steatotic livers can be classified as suboptimal and deteriorate
rapidly during hypothermic static preservation, often resulting in graft nonfunction.
肝移植时供肝经常由于脂肪变性而被放弃。脂肪肝在低温保存期间能被迅速分级为低适度的和变质的。肝移植时脂肪肝经常会导致移植物无功能。
Hypothermic machine perfusion (MP) has been introduced for preservation of donor livers instead of cold storage (CS), resulting in superior preservation outcomes.
低温机器灌注保存（MP）供肝已经代替冷保存(CS)，得到了更好的保存结果。
The aim of this study was to compare CS and MP for preservation of the steatotic donor rat liver.
本研究的目的是比较脂肪变性的供体鼠肝在CS和MP中的保存效果。
Liver steatosis was induced in male Wistar rats by a choline-methionine-deficient diet. After 24 hours hypothermic CS using the University of Wisconsin solution (UW) or MP using UW-Gluconate (UW-G), liver damage (liver enzymes, perfusate flow, and hyaluronic acid clearance) and liver function (bile production, ammonia clearance, urea production, oxygen consumption, adenosine triphosphate [ATP]levels) were assessed in an isolated perfused rat liver model.
用胆素－甲硫氨酸缺乏饮食诱导雄性Wistar鼠肝脏脂肪变性。24小时后用威斯康辛大学溶液(UW)行CS或用UW-葡萄糖酸锑钠 (UW-G)行MP，在隔离的鼠肝模型中评估肝细胞损害（肝脏酶、灌流液灌流和透明质酸酶清除率）和肝功能（胆汁生成、氨清除率、尿素清除率、耗氧量、三磷酸腺苷[ATP]水平）。
Furthermore, liver biopsies were visualized by hematoxylin and eosin staining. Animals developed 30 to 60% steatosis.
Livers preserved by CS sustained significantly more damage as compared to MP. Bile production, ammonia clearance, urea production, oxygen consumption, and ATP levels were significantly higher after MP as compared to CS. These results were confirmed by histology.
苏木素与伊红染色观察肝脏活组织检查。30%到60%的动物肝脂肪变性。持续CS保存肝脏比MP保存有更显著的肝细胞损害。MP保存肝脏在胆汁生成、氨清除率、尿素清除率、耗氧量、ATP水平方面明显高于CS保存。此结果被组织学证实。
In conclusion, MP improves preservation results of the steatotic rat liver, as compared to CS. Liver Transpl
总之，MP明显比CS提高了脂肪变性鼠肝的保存效果。
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作者:admin@医学,生命科学 2011-02-23 17:11
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