主页 > 医药生命 >

Asymmetric Division and Lineage Commitment at the Level of H

Asymmetric Division and Lineage Commitment at the Level of Hematopoietic Stem Cells:Inference from Differentiation in Daughter Cell and Granddaughter Cell Pairs

J. Exp. Med. 2004 199: 295-302.

How hematopoietic stem cells (HSCs) commit to a particular lineage is unclear. A high degree of HSC purification enabled us to address this issue at the clonal level. Single-cell transplantation studies revealed that 40% of the CD34-/low, c-Kit+, Sca-1+, and lineage marker- (CD34-KSL) cells in adult mouse bone marrow were able, as individual cells, to reconstitute myeloid and B- and T-lymphoid lineages over the long-term. Single-cell culture showed that >40% of CD34-KSL cells could form neutrophil (n)/macrophage (m)/erythroblast (E)/megakaryocyte (M) (nmEM) colonies. Assuming that a substantial portion of long-term repopulating cells can be detected as nmEM cells within this population, we compared differentiation potentials between individual pairs of daughter and granddaughter cells derived in vitro from single nmEM cells. One of the two daughter or granddaughter cells remained an nmEM cell. The other showed a variety of combinations of differentiation potential. In particular, an nmEM cell directly gave rise, after one cell division, to progenitor cells committed to nm, EM, or M lineages. The probability of asymmetric division of nmEM cells depended on the cytokines used. These data strongly suggest that lineage commitment takes place asymmetrically at the level of HSCs under the influence of external factors.

http://www.jem.org/cgi/content/abstract/199/3/295 Developmental Stage, Phenotype, and Migration Distinguish Naive- and Effector/Memory-like CD4+ Regulatory T Cells

J. Exp. Med. 2004 199: 303-313.

http://www.jem.org/cgi/content/abstract/199/3/303 [标签:content1][标签:content2]

阅读本文的人还阅读:

【drug-news】Discovery Coul

The Right Time for Sex? 享受性

【medical-news】MDCT yields

The Cancel Chinese Traditiona

【科普】Benefit Of Grape

作者:admin@医学,生命科学    2011-08-14 05:14
医学,生命科学网