主页 > 医药生命 >
【Nature】microRNA能诱导蛋白合成的众多变化
Widespread changes in protein synthesis induced by microRNAs
Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown. In parallel, we quantified mRNA levels using microarrays. Here we show that a single miRNA can repress the production of hundreds of proteins, but that this repression is typically relatively mild. A number of known features of the miRNA-binding site such as the seed sequence also govern repression of human protein synthesis, and we report additional target sequence characteristics. We demonstrate that, in addition to downregulating mRNA levels, miRNAs also directly repress translation of hundreds of genes. Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes. 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Widespread changes in protein synthesis induced by microRNAs
microRNAs诱导的蛋白合成的广泛变化
Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs.
对于动物，微RNA (microRNAs，miRNAs)通过抑制转录和（或）诱导下调目标信使RNA来调控基因表达。
It is unknown how much translational control is exerted by miRNAs on a genome-wide scale.
在基因组水平，哪些转录是由微RNA调控的还不为人知。
We used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown.
我们对由微RNA转染或内源性微RNA敲除引起的几千种蛋白质合成的变化用一种新的蛋白组学方法进行了测定。
In parallel, we quantified mRNA levels using microarrays.
与此同时，我们用微数列来测定mRNA的水平。
Here we show that a single miRNA can repress the production of hundreds of proteins, but that this repression is typically relatively mild.
在此，我们发现单个微RNA能够抑制成百上千种蛋白质的合成，但是这种抑制既典型又相对温和。
A number of known features of the miRNA-binding site such as the seed sequence also govern repression of human protein synthesis, and we report additional target sequence characteristics.
诸如种子序列这样的微RNA结合位点的一些已知特性还能调控抑制人蛋白合成，我们报导了一些新的目标序列特性。
We demonstrate that, in addition to downregulating mRNA levels, miRNAs also directly repress translation of hundreds of genes.
我们也证明了，除了通过下调mRNA的水平之外，微RNA也能直接抑制成百上千基因的转录。
Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes.
最后，我们的数据表明，单个的miRNA能够直接或间接的调节由成百上千基因所表达的蛋白的合成。
编译：
微RNA诱导的蛋白合成的广泛变化
对于动物，微RNA (microRNAs，miRNAs)通过抑制转录和（或）诱导下调目标信使RNA来调控基因表达。在基因组水平，哪些转录是由微RNA调控的还不为人知。我们对由微RNA转染或内源性微RNA敲除引起的几千种蛋白质合成的变化用一种新的蛋白组学方法进行了测定。与此同时，我们用微数列来测定mRNA的水平。在此，我们发现单个微RNA能够抑制成百上千种蛋白质的合成，但是这种抑制既典型又相对温和。诸如种子序列这样的微RNA结合位点的一些已知特性还能调控抑制人蛋白合成，我们报导了一些新的目标序列特性。我们也证明了，除了通过下调mRNA的水平之外，微RNA也能直接抑制成百上千基因的转录。最后，我们的数据表明，单个的微RNA能够直接或间接的调节由成百上千基因所表达的蛋白的合成。 这个比较有意思 [标签:content1][标签:content2]
阅读本文的人还阅读：
作者:admin@医学,生命科学 2011-07-21 18:16
医学,生命科学网