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【Oncogene编译】研究证实DNA损伤压力对p53依赖元件
Stress-specific signatures: expression profiling of p53 wild-type and -null human cells
来源
Oncogene advance online publication 11 April 2005;
doi:10.1038/sj.onc.1208653
http://www.nature.com/cgi-taf/dynapage.taf?file=/onc/journal/vaop/ncurrent/abs/1208653a.html
原题
Gene expression responses of human cell lines exposed to a diverse set of stress agents were compared by cDNA microarray hybridization. The B-lymphoblastoid cell line TK6 (p53 wild-type) and its p53-null derivative, NH32, were treated in parallel to facilitate investigation of p53-dependent responses. RNA was extracted 4 h after the beginning of treatment when no notable decrease in cell viability was evident in the cultures. Gene expression signatures were defined that discriminated between four broad general mechanisms of stress agents: Non-DNA-damaging stresses (heat shock, osmotic shock, and 12-O-tetradecanoylphorbol 13-acetate), agents causing mainly oxidative stress (arsenite and hydrogen peroxide), ionizing radiations (neutron and γ-ray exposures), and other DNA-damaging agents (ultraviolet radiation, methyl methanesulfonate, adriamycin, camptothecin, and cis-Platinum(II)diammine dichloride (cisplatin)). Within this data set, non-DNA-damaging stresses could be discriminated from all DNA-damaging stresses, and profiles for individual agents were also defined. While DNA-damaging stresses showed a strong p53-dependent element in their responses, no discernible p53-dependent responses were triggered by the non-DNA-damaging stresses. A set of 16 genes did exhibit a robust p53-dependent pattern of induction in response to all nine DNA-damaging agents, however.
编译
通过cDNA微阵列杂交比较人细胞系暴露在不同压力下的基因表达效应。B-类淋巴细胞系TK6 (p53 野生型)和它的P53缺乏的衍生系NH32在平行条件下处理，以便于研究其随p53变化的效应。开始处理后，在培养细胞中细胞生存能力没有明显降低的情况下，提取RNA4小时。在四种广泛存在的产生压力物质中观察基因表达信号：非DNA损伤压力（热休克，渗透休克和12-氧-十四烷酰戟二萜醇乙酸酯(致癌剂)）；主要引起氧化损伤的药剂（亚砷酸和过氧化氢）；电离辐射（中子和γ射线）；其他DNA损伤物质（紫外线，甲磺酸甲酯，喜树碱和顺铂）。在观察的结果中非DNA损伤压力的效果与其他所有DNA损伤压力的效果完全不同。DNA损伤压力对p53依赖元件具有极强的反应，但非DNA损伤压力触发的p53关联效应却很难观测到。 [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-04-06 17:16
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