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【文摘发布】电感耦合等离子体质谱和串联质谱
Author：Tiffany H. Cao, Rita A. Cooney, Michelle M. Woznichak, Sheldon W. May, and Richard F. Browner
Resource：Analytical Chemistry, 2001，73 (13)：2898-2902.
Abstract：A method for speciation and identification of organoselenium metabolites found in human urine samples using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS) and tandem mass spectrometry (MS/MS) is described. Reversed-phase chromatographic separation was used for sample fractionation with the ICP-MS functioning as an element-selective detector, and six distinct selenium-containing species were detected in a human urine sample. Fractions were then collected and analyzed using a triple quadrupole mass spectrometer with electrospray ionization and collision-induced dissociation to obtain structural information. The first two fractions were identified specifically as selenomethionine and selenocystamine, estimated to be present at approximately 11 and 40 ppb, respectively. To the best of our knowledge, this is the first time these two metabolites have been positively identified in human urine.
PMID: 11467533 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Title：Speciation and Identification of Organoselenium Metabolites in Human Urine Using Inductively Coupled Plasma Mass Spectrometry and Tandem Mass Spectrometry
标题：电感耦合等离子体质谱和串联质谱法区分和鉴定人尿中有机硒代谢物
Author：Tiffany H. Cao, Rita A. Cooney, Michelle M. Woznichak, Sheldon W. May, and Richard F. Browner
作者：Tiffany H. Cao, Rita A. Cooney, Michelle M. Woznichak, Sheldon W. May, and Richard F. Browner
Resource：Analytical Chemistry, 2001，73 (13)：2898-2902.
文章来源：分析化学, 2001，73 (13)：2898-2902.
Abstract：A method for speciation and identification of organoselenium metabolites found in human urine samples using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS) and tandem mass spectrometry (MS/MS) is described.
摘要：
描述使用高效液相色谱/电感耦合等离子体质谱(HPLC/ICP-MS)和串联质谱法(MS/MS)区分和鉴定人尿中有机硒代谢物的方法。
Reversed-phase chromatographic separation was used for sample fractionation with the ICP-MS functioning as an element-selective detector, and six distinct selenium-containing species were detected in a human urine sample.
通过ICP-MS做元素选择检测器，联合反相色谱用于样品的分离，六种不同含硒成分在人尿样品中被检出。
Fractions were then collected and analyzed using a triple quadrupole mass spectrometer with electrospray ionization and collision-induced dissociation to obtain structural information.
然后收集成分，使用带电喷雾离子阱和碰撞诱导解离功能的三级四极杆串联质谱来获得结构信息。
The first two fractions were identified specifically as selenomethionine and selenocystamine, estimated to be present at approximately 11 and 40 ppb, respectively.
头两个成分做为硒代甲硫氨酸和硒代胱胺被明确鉴别出来，预计含量大约分别为11 和 40 ppb。
To the best of our knowledge, this is the first time these two metabolites have been positively identified in human urine.
就我们所掌握的最好知识，这是两个代谢物第一次在人尿中被明确鉴别出来。
PMID: 11467533 编译：
电感耦合等离子体质谱和串联质谱法区分和鉴定人尿中有机硒代谢物
Tiffany H. Cao等研究人员发表在《分析化学》上的一篇论文描述了使用高效液相色谱/电感耦合等离子体质谱(HPLC/ICP-MS)和串联质谱法(MS/MS)区分和鉴定人尿中有机硒代谢物的方法。（Analytical Chemistry, 2001，73 (13)：2898-2902.）
这种方法通过ICP-MS做元素选择检测器，联合反相色谱用于样品的分离，使得六种不同含硒成分在人尿样品中被检出。然后收集成分，使用带电喷雾离子阱和碰撞诱导解离功能的三级四极杆串联质谱来获得结构信息。头两个成分做为硒代甲硫氨酸和硒代胱胺被明确鉴别出来，预计含量大约分别为11 和 40 ppb。就我们所掌握的信息，这是两个代谢物第一次在人尿中被明确鉴别出来。（丁香）
中文字符：262 感谢ginnyran！ [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2011-03-24 18:59
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