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【NEJM】Dicer, Drosha与卵巢癌患者的预后相关性
ABSTRACT
Background We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer.
Methods We measured messenger RNA (mRNA) levels of Dicer and Drosha in specimens of invasive epithelial ovarian cancer from 111 patients, using a quantitative reverse-transcriptase–polymerase-chain-reaction assay, and compared the results with clinical outcomes. Validation was performed with the use of published microarray data from cohorts of patients with ovarian, breast, and lung cancer. Mutational analyses of genomic DNA from the Dicer and Drosha genes were performed in a subgroup of ovarian-cancer specimens. Dicer-dependent functional assays were performed by means of in vitro transfection with small interfering RNA (siRNA) and short hairpin RNA (shRNA).
Results Levels of Dicer and Drosha mRNA correlated with the levels of expression of the corresponding protein and were decreased in 60% and 51% of ovarian-cancer specimens, respectively. Low Dicer expression was significantly associated with advanced tumor stage (P=0.007), and low Drosha expression with suboptimal surgical cytoreduction (P=0.02). Cancer specimens with both high Dicer expression and high Drosha expression were associated with increased median survival (>11 years, vs. 2.66 years for other subgroups; P<0.001). We found three independent predictors of reduced disease-specific survival in multivariate analyses: low Dicer expression (hazard ratio, 2.10; P=0.02), high-grade histologic features (hazard ratio, 2.46; P=0.03), and poor response to chemotherapy (hazard ratio, 3.95; P<0.001). Poor clinical outcomes among patients with low Dicer expression were validated in additional cohorts of patients. Rare missense mutations were found in the Dicer and Drosha genes, but their presence or absence did not correlate with the level of expression. Functional assays indicated that gene silencing with shRNA, but not siRNA, may be impaired in cells with low Dicer expression.
Conclusions Our findings indicate that levels of Dicer and Drosha mRNA in ovarian-cancer cells have associations with outcomes in patients with ovarian cancer.
http://intl-content.nejm.org/cgi/content/abstract/359/25/2641 本人已认领该文翻译，48小时后若未提交译文，请其他战友自由认领 Dicer、Drosha酶与卵巢癌患者的预后
ABSTRACT
Background We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer.
背景：我们研究了卵巢癌患者中RNA干扰机制中的关键酶Dicer和Drosha酶
Methods We measured messenger RNA (mRNA) levels of Dicer and Drosha in specimens of invasive epithelial ovarian cancer from 111 patients, using a quantitative reverse-transcriptase–polymerase-chain-reaction assay, and compared the results with clinical outcomes. Validation was performed with the use of published microarray data from cohorts of patients with ovarian, breast, and lung cancer. 红字部分还请各位战友指正，特别是“suboptimal surgical cytoreduction ”，感觉应该是不能行外科手术治疗的意思，但具体怎么翻译成书面语真的很绕口。 击水三千里 战友虽为丁香园新手，但文章翻译得不错！翻译功底可见一斑。以后多多参与到我们动态版战友大家庭的翻译和讨论当中来哈！大家在讨论中共同提高！
suboptimal surgical cytoreduction
surgical cytoreduction 确切含义应为减瘤术，与根治术相对而言，即非百分百消灭肿瘤，仅仅是减少肿瘤负荷。
optimal surgical cytoreduction 含义为理想的或相对比较彻底的减瘤术。
sub是英文里常见的前缀，有以下几个意思
1，表示在某某之下的，下面的，潜在的，例如subway地铁,submarine潜水艇,subsoil底层土,subconscious潜意识。
2，表示级别上低一等的，副的，附属的，例如subeditor副编辑,subcontinent次大陆，subsonic亚音速,subtitle副标题，subplot（小说）中次要的情节。
故本人意见：suboptimal surgical cytoreduction 译为不彻底的/不理想的减瘤术比较合适。如果放在文中，结合上下文为“减瘤术的不彻底性”比较合适。
欢迎战友批评指正！ suboptimal surgical cytoreduction
surgical cytoreduction 确切含义应为减瘤术，与根治术相对而言，即非百分百消灭肿瘤，仅仅是减少肿瘤负荷。
optimal surgical cytoreduction 含义为理想的或相对比较彻底的减瘤术。
sub是英文里常见的前缀，有以下几个意思
1，表示在某某之下的，下面的，潜在的，例如subway地铁,submarine潜水艇,subsoil底层土,subconscious潜意识。
2，表示级别上低一等的，副的，附属的，例如subeditor副编辑,subcontinent次大陆，subsonic亚音速,subtitle副标题，subplot（小说）中次要的情节。
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作者:admin@医学,生命科学 2011-02-25 17:11
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