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[交流]Approaches for the sequence-specific knockdown of mRN

Over the past 25 years there have been thousands of published reports describing applications of antisense nucleic acid derivatives for targeted inhibition of gene function. The major classes of antisense agents currently used by investigators for sequence-specific mRNA knockdowns are antisense oligonucleotides (ODNs), ribozymes, DNAzymes and RNA interference (RNAi). Whatever the method, the problems for effective application are remarkably similar: efficient delivery, enhanced stability, minimization of off-target effects and identification of sensitive sites in the target RNAs. These challenges have been in
existence from the first attempts to use antisense research tools, and need to be met before any antisense molecule can become widely accepted as a therapeutic agent.

过去的25年中，有成千上万的文章发表，描述了反义核酸在抑制目标基因的功能方面的应用。现在被广泛应用的序列特异性的mRNA knockdown的反义技术主要有：反义寡核苷酸（antisense oligonucleotides (ODNs)），核酶（ribozymes），DNA酶（DNAzymes）和干扰RNA（RNA interference (RNAi)）。不管什么方法，影响实际应用效率的问题却是相似的，而这些问题也是反义核酸技术推广应用所必需面临的问题。本文作者对这些方法的原理及遇到的问题进行了综述。
原文: http://www.biosino.org/hotspot/nbt915.pdf

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作者:admin@医学,生命科学    2011-02-16 05:17
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