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【bio-news】iPSC 得分卡预示分化潜力有多大
iPSC 得分卡预示分化潜力有多大
Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines
Authors
Christoph Bock, Evangelos Kiskinis, Griet Verstappen, Hongcang Gu, Gabriella Boulting, Zachary D. Smith, Michael Ziller, Gist F. Croft, Mackenzie W. Amoroso, Derek H. Oakley, Andreas Gnirke, Kevin Eggan, Alexander Meissner
Broad Institute, Cambridge, MA 02142, USA
Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
Harvard Stem Cell Institute, Cambridge, MA 02138, USA
Max Planck Institute for Informatics, 66123 Saarbrücken, Germany
The Howard Hughes Medical Institute, USA
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Departments of Pathology, Neurology, and Neuroscience, Center for Motor Neuron Biology and Disease (MNC), and Columbia Stem Cell Initiative (CSCI), Columbia University, New York, NY 10032, USA
Corresponding author
Highlights
?Genome-wide maps of DNA methylation and gene expression for 32 ES and iPS cell lines
?Some but not all iPS cell lines fall within the spectrum of observed ES cell variation
?Epigenetic and transcriptional defects can interfere with in vitro differentiation
?High-throughput assay quantifies cell-line-specific differentiation propensities
Summary
The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
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作者:admin@医学,生命科学 2011-02-16 00:03
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