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【科普】美科学家发现一种小核糖核酸有助治疗
卵巢癌往往到晚期才被发现，而等到发现时，癌细胞已经转移到其他器官，难以通过化疗等方法治疗。美国最新研究发现，一种小核糖核酸可以有效对抗卵巢癌，这一发现将使医生有可能采用传统的化疗方法治疗卵巢癌。
美国佐治亚理工学院的研究人员在美国新一期《妇科肿瘤学》杂志上报告说，他们在实验中利用具有调控功能的小核糖核酸－429，使卵巢癌细胞转变成不能转移的状态，从而使癌细胞无法扩散。
负责这项研究的约翰·麦克唐纳指出，这一发现将使医生有可能将病人的癌细胞生命周期调回到可以采用传统的化疗方法治疗的阶段。 Overexpression of miR-429 induces mesenchymal-to-epithelial transition (MET) in metastatic ovarian cancer cells
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Jing Chena, Lijuan Wanga, Lilya V. Matyuninaa, Christopher G. Hilla and John F. McDonald, a,
a Integrated Cancer Research Center, School of Biology and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA 30332-0363, USA
Received 9 August 2010. Available online 28 January 2011.
Abstract
Objective
Ovarian cancer (OC) is the most lethal of all gynecological malignancies primarily due to the sloughing-off of highly metastatic cells from primary tumors and their subsequent spread throughout the peritoneal cavity. Since the epithelial-to-mesenchymal transition (EMT) of OC cells located at the periphery of primary tumors is essential to this process, molecular interventions that can block EMT are of potential clinical significance. Members of the miR200 family of microRNAs have been implicated in EMT in other cancers. Our purpose was to determine if miR200 family microRNAs may be involved in EMT in OC and of potential therapeutic value in reducing OC metastasis.
Methods
Gene expression profiles of two OC cell lines with different metastatic potentials were monitored using qRT-PCR (quantitative reverse transcription polymerase chain reaction). The effect of over-expression of a miR-200 family microRNA (miR-429) in metastatic OC cells was monitored on molecular (qRT-PCR and microarray) and functional (morphology, migration, invasiveness and anchorage independence assays) levels.
Results
Molecular profiling of two OC cell lines with differing metastatic potentials identified significant differences in previously established epithelial and mesenchymal cell biomarkers including E-cadherin, ZEB1, ZEB2, miR-205 and miR-200 family microRNAs. Ectopic overexpression of miR-429, a member of the miR-200 family of microRNAs, in mesenchymal-like OC cells resulted in reversal of the mesenchymal phenotype (mesenchymal–epithelial transition, MET).
Conclusions
Our results indicate that miR-429 may not only be a useful biomarker of EMT in ovarian cancer, but also of potential therapeutic value in abating OC metastasis.
Research Highlights
? miR-429 induces MET in a highly metastatic ovarian cancer cell line. ? Most miR-429 induced molecular changes are indirect. ? miR-429 may be of therapeutic value in reducing OC metastasis and tumor recurrence. 自然科学总论(理论、现状及发展)文件类型:PDF/Adobe Acrobat- 文字版新型 X 射线探测器的研制等,都是一种挑战,也是一个国家科学技术总体水 平的体现. ... (mitochondrion)的脱氧核糖核酸(DNA).他们发现不同类型的线粒体 DNA, 有些互相接近...www.clzx.cquc.edu.cn/...68.pdf-2011-01-30-科学总论(理论、现状及发展)" 二千沙龙社区-[分享]健康小知识(不断更新)近年来,随着医药科学的发展,专家们发现许多药物的疗效与用药时间密切相关.这是因为... 香港武术师佛山助残献爱心 弘扬华夏医药文化,建设特色科普基地 http://fm001.cn/...www.c2000.cn/...D=84&id=150778-2005-08-26-快照-北京师范大学图书馆 生命科学图书分馆【篇名】 国外生物信息学发展动态分析 【作者】王玉梅.王艳. 【关键词】 生命科学.生... 达到靶向诊断、治疗的目的.结论:叶酸受体介导的靶向给药是一种很有前景的给药方式. ...202.112.82.59/...ang/xkzht.htm-2011-01-21-快照-生命科学图书分馆"[标签:content2]
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作者:admin@医学,生命科学 2011-02-07 12:02
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