主页 > 医学新闻 >
【medical-news】福尔马林固定的肿瘤组织中发现新
Stanford, CA — Researchers led by Dr. Soheil Dadras at the Stanford University Medical Center have developed a novel methodology to extract microRNAs from cancer tissues. The related report by Ma et al, "Profiling and discovery of novel miRNAs from formalin-fixed paraffin-embedded melanoma and nodal specimens," appears in the September 2009 issue of the Journal of Molecular Diagnostics.
Cancer tissues from patients are often stored by a method that involves formalin fixation and paraffin embedding to retain morphological definition for identification; however, this method frequently prevents further molecular analysis of the tissue because of mRNA degradation. Even so, these tissues contain high numbers of microRNAs (miRNAs), which are short enough (~22 nucleotides) to not be broken down during the fixation process.
In this study, Dr. Dadras and colleagues optimized a new protocol for extracting miRNAs from formalin-fixed paraffin-embedded tissues. Using their new procedure, they identified 17 new and 53 known miRNAs from normal skin, melanoma, and sentinel lymph nodes. These miRNAs were well-preserved in a 10-year-old specimen. This new protocol, therefore, will allow for the identification of novel miRNAs that may differ in cancerous and healthy tissue, even from long-preserved tissue, leading to better predictions of disease prognosis and treatment response.
Ma et al suggest that their "cloning strategy has the advantage of not only discovering novel and known miRNA sequence identity but also providing an estimate of relative expression level. … [This methodology may provide] a more robust strategy to obtain an accurate expression profile for novel and/or previously characterized small RNAs from clinically defined [formalin-fixed paraffin-embedded] tumor specimens, thereby facilitating the discovery of 'oncomirs' as biomarkers." 自己认领了，24h翻译 Stanford, CA — Researchers led by Dr. Soheil Dadras at the Stanford University Medical Center have developed a novel methodology to extract microRNAs from cancer tissues. The related report by Ma et al, "Profiling and discovery of novel miRNAs from formalin-fixed paraffin-embedded melanoma and nodal specimens," appears in the September 2009 issue of the Journal of Molecular Diagnostics.
Stanford, CA — 斯坦福大学医学中心Dr. Soheil Dadras研究小组研究出了从肿瘤组织中提取microRNAs的新方法。Ma等人的相关报告，“从福尔马林固定石蜡包埋的黑素瘤和淋巴结标本中剖析发现了新的miRNAs，”发表在2009年9月的Journal of Molecular Diagnostics杂志。
Cancer tissues from patients are often stored by a method that involves formalin fixation and paraffin embedding to retain morphological definition for identification; however, this method frequently prevents further molecular analysis of the tissue because of mRNA degradation. Even so, these tissues contain high numbers of microRNAs (miRNAs), which are short enough (~22 nucleotides) to not be broken down during the fixation process.
病人中的癌组织经常用福尔马林固定石蜡包埋方法储存从而保持形态便于辨认定义。然而，这种方法经常破坏进一步的组织分子分析，因为mRNA被降解了。尽管如此，这些组织仍然有大量的miRNAs，因其短（22个核苷酸）不被福尔马林破坏。
In this study, Dr. Dadras and colleagues optimized a new protocol for extracting miRNAs from formalin-fixed paraffin-embedded tissues. Using their new procedure, they identified 17 new and 53 known miRNAs from normal skin, melanoma, and sentinel lymph nodes. These miRNAs were well-preserved in a 10-year-old specimen. This new protocol, therefore, will allow for the identification of novel miRNAs that may differ in cancerous and healthy tissue, even from long-preserved tissue, leading to better predictions of disease prognosis and treatment response.
在研究中，Dr. Dadras 和他的同事优化了一种新的从福尔马林固定石蜡包埋组织中提取miRNAs的新方法。通过新方法，他们确认了17中新的53中已知的来自皮肤，黑素瘤和前哨淋巴结的miRNAs。这些miRNAs一直完好保存在10年的样本中。这种新的技术便于辨认癌组织和正常组织中不同的新的miRNAs，即使是来自长时间保存的组织，这样就能更好的预测疾病预后情况和治疗反应。
Ma et al suggest that their "cloning strategy has the advantage of not only discovering novel and known miRNA sequence identity but also providing an estimate of relative expression level. … [This methodology may provide] a more robust strategy to obtain an accurate expression profile for novel and/or previously characterized small RNAs from clinically defined [formalin-fixed paraffin-embedded] tumor specimens, thereby facilitating the discovery of 'oncomirs' as biomarkers."
Ma等人建议他们的“克隆方法不仅可以发现新的和已经的miRNA的序列一致性还可以估计相对表达水平。...这种方法提供了更健全的获得新的/或者以前临床上确认的sRNAs正确的表达水平。有助于‘oncomirs’作为生物标记的发现。 Stanford, CA — 斯坦福大学医学中心Dr. Soheil Dadras研究小组研究出了从肿瘤组织中提取microRNAs的新方法。Ma等人的相关报告，“从福尔马林固定石蜡包埋的黑素瘤和淋巴结标本中剖析发现了新的miRNAs，”发表在2009年9月的Journal of Molecular Diagnostics杂志。
阅读本文的人还阅读：
作者:admin@医学,生命科学 2010-09-26 05:11
医学,生命科学网