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【文摘发布】分子印迹聚合物柱提取、液相色谱
Author：Yang Xia, James E. McGuffey, Sumita Bhattacharyya, Börje Sellergren, Ecevit Yilmaz, Lanqing Wang, and John T. Bernert .
Resource: Analytical Chemistry, 2005,77 (23)： 7639 -7645.
Abstract:
The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) is present in the urine of tobacco users and, at lower concentrations, in the urine of nonsmokers exposed to secondhand smoke. NNAL is a valuable biomarker of human exposure to the carcinogenic nitrosamines in tobacco and tobacco smoke, but its presence at low concentrations in urine requires sensitive and often complex analytic procedures. In this report, we describe the development of an efficient method for the analysis of NNAL in human urine using liquid chromatography/atmospheric pressure ionization tandem mass spectrometry (LC/MS/MS) combined with a novel sample cleanup based on a molecularly imprinted polymer (MIP) column developed specifically for this assay. Our results suggest that this combination of MIP column extraction and LC/MS/MS can provide a sensitive and relatively simple analytical method suitable for application to epidemiologic investigations of health risks associated with the exposure to tobacco smoke or SHS in both smokers and nonsmokers. 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Title：Analysis of the Tobacco-Specific Nitrosamine 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Urine by Extraction on a Molecularly Imprinted Polymer Column and Liquid Chromatography/Atmospheric Pressure Ionization Tandem Mass Spectrometry 题目：分子印迹聚合物柱提取、液相色谱/大气压电离串联质谱法分析尿液中烟草特有的亚硝胺4-(甲基亚硝胺)-1-(3-吡啶)-1-丁醇
Author：Yang Xia, James E. McGuffey, Sumita Bhattacharyya, Börje Sellergren, Ecevit Yilmaz, Lanqing Wang, and John T. Bernert .
作者：Yang Xia, James E. McGuffey, Sumita Bhattacharyya, Börje Sellergren, Ecevit Yilmaz, Lanqing Wang, and John T. Bernert .
Resource: Analytical Chemistry, 2005,77 (23)： 7639 -7645.
来源：分析化学，2005，77（23）：7639 -7645.
Abstract:
摘要：
The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) is present in the urine of tobacco users and, at lower concentrations, in the urine of nonsmokers exposed to secondhand smoke. NNAL is a valuable biomarker of human exposure to the carcinogenic nitrosamines in tobacco and tobacco smoke, but its presence at low concentrations in urine requires sensitive and often complex analytic procedures. In this report, we describe the development of an efficient method for the analysis of NNAL in human urine using liquid chromatography/atmospheric pressure ionization tandem mass spectrometry (LC/MS/MS) combined with a novel sample cleanup based on a molecularly imprinted polymer (MIP) column developed specifically for this assay. Our results suggest that this combination of MIP column extraction and LC/MS/MS can provide a sensitive and relatively simple analytical method suitable for application to epidemiologic investigations of health risks associated with the exposure to tobacco smoke or SHS in both smokers and nonsmokers.
烟草特有的亚硝胺4-(甲基亚硝胺)-1-(3-吡啶)-1-丁醇（NNAL）存在于吸烟者和被动吸二手烟的非吸烟者人群的尿液中，只是被动吸二手烟的非吸烟者人群尿液中的浓度更小。NNAL是一种很有价值的生物标志物，它存在于接触烟草或烟气中致癌物质——N－亚硝胺的人群体内，但是它在尿液中浓度很低，（使得检测NNAL）往往需要灵敏而又复杂的检测分析方法。在这篇报告中，我们描述了一种可以有效检测人体尿液中NNAL的新方法的研制过程，该方法使用液相色谱/高压电离质谱联用法（LC/MS/MS），与一种新的样品净化方法结合，此样品净化方法基于本分析特有的分子印迹聚合物（MIP）柱。我们的结果表明，联合分子印迹聚合物（MIP）柱和LC/MS/MS法可以提供一种灵敏而相对简单的分析方法，该分析方法适合对吸烟者和非吸烟者由于接触烟气或SHS引起的健康威胁进行流行病学调查。
编译：
题目：分子印迹聚合物柱提取、液相色谱/大气压电离串联质谱法分析尿液中烟草特有的亚硝胺4-(甲基亚硝胺)-1-(3-吡啶)-1-丁醇
作者：Yang Xia, James E. McGuffey, Sumita Bhattacharyya, Börje Sellergren, Ecevit Yilmaz, Lanqing Wang, and John T. Bernert .
来源：分析化学，2005，77（23）：7639 -7645.
摘要：
烟草特有的亚硝胺4-(甲基亚硝胺)-1-(3-吡啶)-1-丁醇（NNAL）存在于吸烟者和被动吸二手烟的非吸烟者人群的尿液中，只是被动吸二手烟的非吸烟者人群尿液中的浓度更小。NNAL是一种很有价值的生物标志物，它存在于接触烟草或烟气中致癌物质——N－亚硝胺的人群体内，但是它在尿液中浓度很低，（使得检测NNAL）往往需要灵敏而又复杂的检测分析方法。在这篇报告中，我们描述了一种可以有效检测人体尿液中NNAL的新方法的研制过程，该方法使用液相色谱/高压电离质谱联用法（LC/MS/MS），与一种新的样品净化方法结合，此样品净化方法基于本分析特有的分子印迹聚合物（MIP）柱。我们的结果表明，联合分子印迹聚合物（MIP）柱和LC/MS/MS法可以提供一种灵敏而相对简单的分析方法，该分析方法适合对吸烟者和非吸烟者由于接触烟气或SHS引起的健康威胁进行流行病学调查。 感谢chhao!
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作者:admin@医学,生命科学 2011-03-10 05:12
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