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【文摘发布】HLA特异性B细胞：用HLA四聚体的定性
Source：Transplantation. 2007 Apr 15;83(7):982-8
Abstract：BACKGROUND: The development of highly sensitive and specific assays for detecting and characterizing HLA-specific antibodies has contributed to an appreciation of the extensive involvement of those antibodies in graft injury and dysfunction. However, understanding the regulatory processes of the humoral response to transplantation and the mechanisms underlying therapeutic agents and protocols for preventing and treating sensitization requires a way to study HLA-specific B cells. METHODS: Lymphocyte preparations enriched for B cells were stained with one or more of three different HLA tetramers. Tetramer-positive (tet+) B cells were enumerated and evaluated for an association of their frequencies with known sensitization. In some cases, tet+ B cells were isolated and placed in culture with supplements known to activate B cells in a nonspecific fashion. RESULTS: For all tetramers used, the frequencies of tet+ B cells were significantly higher (4.1%-5.5%) among sensitized patients than among nonsensitized patients (1.6%-3.2%, P<0.001). Binding of the tetramers occurred by the surface immunoglobulin antigen receptor with little or no binding to antibody captured in the Fc receptor. Cultured tet+ B cells produced antibodies specific for epitopes of the tetramer antigen. There appeared to be a certain amount of crossreactivity in the binding of tetramers. The frequency of CD27+ cells among tet+ B cells was higher, on average (34.4%-38.8%) than among all B cells (26.2%) whereas the frequencies of CD38 were comparable in the two groups. CONCLUSIONS: Staining with HLA tetramers provides a means for identifying, quantifying, and isolating HLA-specific B cells.
PMID: 17460571 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领 Title：HLA-specific B cells: I. A method for their detection, quantification, and isolation using HLA tetramers.
HLA特异性B细胞：一种借助于HLA四聚体对其进行的定性、定量和分离方法
Author：Alakulppi NS, Kyllonen LE, Partanen J, Salmela KT, Laine JT.
作者：Alakulppi NS, Kyllonen LE, Partanen J, Salmela KT, Laine JT.
Source：Transplantation. 2007 Apr 15;83(7):982-8
来源：移植杂志 期号:2007 年4月15日:83(7):982-8
Abstract：摘要
BACKGROUND: 研究现状
The development of highly sensitive and specific assays for detecting and characterizing HLA-specific antibodies has contributed to an appreciation of the extensive involvement of those antibodies in graft injury and dysfunction.
高敏感性及特异性的用以鉴定并将HLA特异性抗体特征化的分析技术的发展归功于针对广泛的涉及该类抗体在移植物损伤和功能障碍方面研究。
However, understanding the regulatory processes of the humoral response to transplantation and the mechanisms underlying therapeutic agents and protocols for preventing and treating sensitization requires a way to study HLA-specific B cells.
但是，要了解针对移植的体液反应的调节进程和隐在为预防和处置致敏作用而采取的多种治疗因素及方案之下的机制，需要一种用以研究HLA特异性B细胞的方法
METHODS:方法
Lymphocyte preparations enriched for B cells were stained with one or more of three different HLA tetramers. Tetramer-positive (tet+) B cells were enumerated and evaluated for an association of their frequencies with known sensitization. In some cases, tet+ B cells were isolated and placed in culture with supplements known to activate B cells in a nonspecific fashion.
为获取B细胞而对浓缩的淋巴细胞样品采用从三种不同的HLA 四聚物中选出的1种或更多进行染色。四聚物阳性(即tet+)的B细胞被借助其显现频率与已知的致敏作用的相互关联而计数和评估。在某些实验中，四聚物阳性B细胞被分离出来并被放置在含有可以非特异性方式激活B细胞的补体的培养基内。
RESULTS: For all tetramers used, the frequencies of tet+ B cells were significantly higher (4.1%-5.5%) among sensitized patients than among nonsensitized patients (1.6%-3.2%, P<0.001). Binding of the tetramers occurred by the surface immunoglobulin antigen receptor with little or no binding to antibody captured in the Fc receptor.
结果：对所应用的所有四聚物而言，四聚物阳性B细胞在致敏患者中的出现频率明显高(4.1%-5.5%)于非致敏患者(1.6%-3.2%, P<0.001)中的出现率。四聚物的结合是通过表面免疫球蛋白抗原受体借助于Fc受体与少量或者没有俘获过抗体的结合实现的。
Cultured tet+ B cells produced antibodies specific for epitopes of the tetramer antigen. There appeared to be a certain amount of crossreactivity in the binding of tetramers. The frequency of CD27+ cells among tet+ B cells was higher, on average (34.4%-38.8%) than among all B cells (26.2%) whereas the frequencies of CD38 were comparable in the two groups.
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作者:admin@医学,生命科学 2011-03-12 23:54
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