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【文摘发布】表面活性剂聚羟亚烃188在鼠颅内出
intracranial hemorrhage in rats.
Author：Cadichon SB, Le Hoang M, Wright DA, Curry DJ, Kang U, Frim DM.
Resource: J Neurosurg. 2007 Jan;106(1 Suppl):36-40.
Abstract：OBJECT: Neuronal injury remains a leading cause of morbidity in both neonates
and adults with injuries induced by intracranial hemorrhage,
ischemia-reperfusion, and excitotoxicity. To date, a number of neuroprotective
strategies have been evaluated, but they have shown little benefit. Poloxamer
188 (P-188), a membrane-active triblock copolymer, has been studied extensively
as a cell-membrane sealant. The authors used an animal model to study the
neuroprotectant effects of P-188 administered by intracisternal (IC) injection
after experimentally induced intraparenchymal hemorrhage. METHODS:
Sprague-Dawley rats received an IC injection of either P-188 or vehicle
(artificial cerebrospinal fluid) 10 minutes after striatal infusion of 50 microl
of autologous blood. Animals from both treatment groups were killed either 2 or
7 days later. In a second experiment, after striatal blood infusion and early IC
injection of either P-188 or vehicle, animals received daily IC injections of
either P- 188 or vehicle for 5 days, and were killed 7 days after induction of
the experimental hemorrhage. Striatal tissues were histologically analyzed for
neuronal loss, and lesion volumes were determined. Lesion volumes in the animals
that received a single dose of P-188 were significantly smaller (mean+/-standard
deviation 18.3+/-4.3 mm(3), six rats; p = 0.04) than those in the control group
(31.4+/-4.3 mm(3), seven rats) when measured 2 days postinjection; however, no
difference in lesion volumes was present 7 days postinjection. Lesion volumes in
the animals who received 5 days of daily P-188 injections were significantly
smaller (1.50+/-0.58 mm(3), 10 rats; p = 0.04) than those in the corresponding
control group (5.04+/-1.85 mm(3), eight rats) when measured at 7 days.
CONCLUSIONS: A single dose of P- 188 protects against early neuronal loss after
hemorrhage but has no effect on long-term hemorrhage-induced neuronal loss.
However, repeated daily P-188 treatment appears to produce effective long-term
neuronal protection.
PMID: 17233310 本人已认领该文编译，48小时后若未提交译文，请其他战友自由认领。 Title：Neuroprotective effect of the surfactant poloxamer 188 in a model of
intracranial hemorrhage in rats.
题目：表面活性剂聚羟亚烃188在鼠颅内出血后的神经保护作用
Author：Cadichon SB, Le Hoang M, Wright DA, Curry DJ, Kang U, Frim DM.
作者：Cadichon SB, Le Hoang M, Wright DA, Curry DJ, Kang U, Frim DM.
Resource: J Neurosurg. 2007 Jan;106(1 Suppl):36-40.
来源：J Neurosurg. 2007 Jan;106(1 Suppl):36-40.
Abstract：OBJECT: Neuronal injury remains a leading cause of morbidity in both neonates and adults with injuries induced by intracranial hemorrhage,ischemia-reperfusion, and excitotoxicity. 摘要：目的：神经元损伤是新生儿和成年人颅内出血、缺血再灌注和兴奋性毒性致死的主要原因。To date, a number of neuroprotective strategies have been evaluated, but they have shown little benefit. 迄今为止，有很多的神经保护对策被验证过，但是这些方法只有微弱的作用。Poloxamer 188 (P-188), a membrane-active triblock copolymer, has been studied extensively as a cell-membrane sealant. 表面活性剂聚羟亚烃188（P-188）是一种膜活性的三嵌段共聚物，作为细胞膜的封闭剂已被广泛的研究。The authors used an animal model to study the
neuroprotectant effects of P-188 administered by intracisternal (IC) injection after experimentally induced intraparenchymal hemorrhage. 作者利用动物模型在实验性脑实质内出血后脑池内注射P188研究P188的神经保护作用。METHODS: Sprague-Dawley rats received an IC injection of either P-188 or vehicle (artificial cerebrospinal fluid) 10 minutes after striatal infusion of 50 microl of autologous blood. 方法：SD大鼠在纹状体注入50μl自体血液10分钟后接受脑池内注射P188或对照（人工脑脊液）。 Animals from both treatment groups were killed either 2 or 7 days later. 两处理组动物在2天或7天后处死。In a second experiment, after striatal blood infusion and early IC injection of either P-188 or vehicle, animals received daily IC injections of either P- 188 or vehicle for 5 days, and were killed 7 days after induction of the experimental hemorrhage. 第二个实验中，在纹状体注入血液和脑池内注射P188或人工脑脊液后，动物接受每日脑池内注射P188或对照液连续5天，在实验性脑出血后7天处死。 Striatal tissues were histologically analyzed for neuronal loss, and lesion volumes were determined. 纹状体用来做神经元缺失的组织学分析，同时评价损伤面积。Lesion volumes in the animals that received a single dose of P-188 were significantly smaller (mean+/-standard deviation 18.3+/-4.3 mm(3), six rats; p = 0.04) than those in the control group (31.4+/-4.3 mm(3), seven rats) when measured 2 days postinjection; 接受单次P188注射动物的损伤面积（均数±标准差=18.3+/-4.3 mm(3)，6只，p = 0.04）明显小于对照组动物(均数±标准差=31.4+/-4.3 mm(3), 7只)。however, no difference in lesion volumes was present 7 days postinjection. 但是在注射后7天两组损伤面积没有差异。Lesion volumes in the animals who received 5 days of daily P-188 injections were significantly smaller (1.50+/-0.58 mm(3), 10 rats; p = 0.04) than those in the corresponding control group (5.04+/-1.85 mm(3), eight rats) when measured at 7 days. 7天后的测量结果表明：接受连续5天注射P188动物的损伤面积(均数±标准差=1.50+/-0.58 mm(3), 10只大鼠; p = 0.04)明显小于相应的对照组(均数±标准差=5.04+/-1.85 mm(3), 8只大鼠)。 CONCLUSIONS: A single dose of P- 188 protects against early neuronal loss after hemorrhage but has no effect on long-term hemorrhage-induced neuronal loss. However, repeated daily P-188 treatment appears to produce effective long-term neuronal protection.结论：单次剂量的P188可保护出血后早期的神经元缺失，但是对于出血导致的长期神经元脱失没有作用。每日重复给予P188对于长期神经元损伤有保护作用。
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作者:admin@医学,生命科学 2011-01-04 05:14
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