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【medical-news】甲基化标记物可有助于识别膀胱癌
纽约（路透社健康新闻）－利用实时PCR方法检测尿沉渣DNA甲基化标记物可有助于检测膀胱癌。研究者的报告发表于7月份的《国立癌症研究所杂志》上。
作为高级调查者，David Sidransky博士告诉路透社健康版记者：“对于膀胱癌病人，我们正在寻找一种简单的尿液检测的方法，而甲基化标记物可提供一个高度的敏感性和特异性。”
Baltimore的Johns Hopkins医学院的Sidransky博士和同事指出，异常的促进子甲基化是人类癌症中肿瘤抑制基因和其它基因沉默的一种主要机制。
To determine whether hypermethylation might be indicative of bladder cancer, the researchers examined primary tumor DNA and urine sediment DNA in 9 genes from 15 patients with bladder cancer and in 25 controls.
为了明确高甲基化是否可提示旁观癌，研究者研究了15例膀胱癌病人和25例对照组中9个基因的原发肿瘤DNA 和尿沉渣DNA。
对于所有15例膀胱癌病人，尿液中促进子甲基化模式与肿瘤一致。研究者发现4个基因对膀胱癌显示100％的特异性。
接着他们检测了160例膀胱癌病人和69例年龄配对的对照组的尿液沉渣DNA样本。
在160例病人，加上原来的15例膀胱癌病人中，121例（69％）病人4个基因至少有1个显示促进子甲基化，而对照组均无这样的甲基化。
研究者发现在没有这些基因有甲基化的病人中，以其它的5个基因为基础，附加上logic回归积分，可使敏感性从69％提高到82％，但特异性从100％下降至96%.
Sidransky博士认为：“除了这种方法告诉我们癌细胞存在外，有一天它可能还告诉我们癌是否处于进展和是否需要其它的治疗。”
Methylation Markers May Help Spot Bladder Cancer
NEW YORK (Reuters Health) Jul 26 - Detecting methylation markers in urine sediment DNA via real-time polymerase chain reaction may help detect bladder cancer, researchers report in the July 19th issue of the Journal of the National Cancer Institute.
As senior investigator Dr. David Sidransky told Reuters Health: "methylation markers may provide the high sensitivity and specificity we have been searching for in the form of a simple urine test for patients with bladder cancer."
Dr. Sidransky of the Johns Hopkins School of Medicine in Baltimore, and colleagues note that aberrant promoter methylation is a major mechanism for silencing tumor-suppressor and other genes in human cancers.
For all 15 patients, the promoter methylation pattern in urine matched that in the tumor. The investigators found that four genes displayed 100% specificity for bladder cancer.
They then examined urine sediment DNA samples from another 160 patients with bladder cancer and 69 age-matched controls.
Of these 160 patients, plus the original 15 bladder cancer patients, 121 (69%) had promoter methylation in at least one of the four genes, whereas no controls showed such methylation.
The researchers found that in patients who did not have methylated versions of these genes, addition of a logical regression score based on the other five genes improved sensitivity from 69% to 82%, but specificity fell from 100% to 96%.
Dr. Sidransky pointed out that "in addition to telling us that cancer cells are present, this approach may one day tell us if the cancer is advanced and if additional treatments are necessary." [标签:content1][标签:content2]
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作者:admin@医学,生命科学 2010-12-27 17:14
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